In vivo determination of transport and metabolism of deoxyglucose in intestinal tissues

J. Hamar, Gyula Hutiray

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Experiments were carried out on 11 anesthetized cats (Na-pentobarbital). Uptake of a glucose analogue (2-deoxy-d-glucose) by intestinal mucosa and muscularis from arterial plasma was studied in order to determine net rate of transport and phosphorylation of the material. The theoretical basis for calculating the rate constants of forward (k 1 x ) and reverse (k 2 x ) transport between plasma and tissue and also phosphorylation (k 3 x ) and dephosphorylation (k 4 x ) of14C labeled deoxy-glucose (DG) was determined. The method can also be used for estimating tissue glucose uptake. The rate constants were found to be:k 1 x =0.669 and 0.873;k 2 x =2.285 and 4.656;k 3 x =0.057 and 0.067;k 4 x =0.091 and 0.097 [s-1] in the mucosa and muscularis, respectively. Glucose utilisation of intestinal mucosa was 2.69 and that of muscularis 2.46 mg/(min×100 g) tissue, respectively. Arterial glucose concentration was constant during the studies, however, it showed a variation from 120 to 250 mg/100 ml of plasma from animal to animal. Tissue glucose uptake or any of the rate constants were not influenced by the plasma level over this range.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalPflugers Archiv European Journal of Physiology
Volume401
Issue number3
DOIs
Publication statusPublished - Jul 1984

Fingerprint

Deoxyglucose
Metabolism
Tissue
Glucose
Plasmas
Rate constants
Phosphorylation
Intestinal Mucosa
Animals
Pentobarbital
Mucous Membrane
Cats

Keywords

  • Glucose consumption
  • Glucose transport
  • Hexokinase
  • Intestinal mucosa
  • Muscularis
  • Phosphatase

ASJC Scopus subject areas

  • Physiology

Cite this

In vivo determination of transport and metabolism of deoxyglucose in intestinal tissues. / Hamar, J.; Hutiray, Gyula.

In: Pflugers Archiv European Journal of Physiology, Vol. 401, No. 3, 07.1984, p. 233-238.

Research output: Contribution to journalArticle

@article{52b7310e594e4a6482b7cb84f61f417f,
title = "In vivo determination of transport and metabolism of deoxyglucose in intestinal tissues",
abstract = "Experiments were carried out on 11 anesthetized cats (Na-pentobarbital). Uptake of a glucose analogue (2-deoxy-d-glucose) by intestinal mucosa and muscularis from arterial plasma was studied in order to determine net rate of transport and phosphorylation of the material. The theoretical basis for calculating the rate constants of forward (k 1 x ) and reverse (k 2 x ) transport between plasma and tissue and also phosphorylation (k 3 x ) and dephosphorylation (k 4 x ) of14C labeled deoxy-glucose (DG) was determined. The method can also be used for estimating tissue glucose uptake. The rate constants were found to be:k 1 x =0.669 and 0.873;k 2 x =2.285 and 4.656;k 3 x =0.057 and 0.067;k 4 x =0.091 and 0.097 [s-1] in the mucosa and muscularis, respectively. Glucose utilisation of intestinal mucosa was 2.69 and that of muscularis 2.46 mg/(min×100 g) tissue, respectively. Arterial glucose concentration was constant during the studies, however, it showed a variation from 120 to 250 mg/100 ml of plasma from animal to animal. Tissue glucose uptake or any of the rate constants were not influenced by the plasma level over this range.",
keywords = "Glucose consumption, Glucose transport, Hexokinase, Intestinal mucosa, Muscularis, Phosphatase",
author = "J. Hamar and Gyula Hutiray",
year = "1984",
month = "7",
doi = "10.1007/BF00582589",
language = "English",
volume = "401",
pages = "233--238",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - In vivo determination of transport and metabolism of deoxyglucose in intestinal tissues

AU - Hamar, J.

AU - Hutiray, Gyula

PY - 1984/7

Y1 - 1984/7

N2 - Experiments were carried out on 11 anesthetized cats (Na-pentobarbital). Uptake of a glucose analogue (2-deoxy-d-glucose) by intestinal mucosa and muscularis from arterial plasma was studied in order to determine net rate of transport and phosphorylation of the material. The theoretical basis for calculating the rate constants of forward (k 1 x ) and reverse (k 2 x ) transport between plasma and tissue and also phosphorylation (k 3 x ) and dephosphorylation (k 4 x ) of14C labeled deoxy-glucose (DG) was determined. The method can also be used for estimating tissue glucose uptake. The rate constants were found to be:k 1 x =0.669 and 0.873;k 2 x =2.285 and 4.656;k 3 x =0.057 and 0.067;k 4 x =0.091 and 0.097 [s-1] in the mucosa and muscularis, respectively. Glucose utilisation of intestinal mucosa was 2.69 and that of muscularis 2.46 mg/(min×100 g) tissue, respectively. Arterial glucose concentration was constant during the studies, however, it showed a variation from 120 to 250 mg/100 ml of plasma from animal to animal. Tissue glucose uptake or any of the rate constants were not influenced by the plasma level over this range.

AB - Experiments were carried out on 11 anesthetized cats (Na-pentobarbital). Uptake of a glucose analogue (2-deoxy-d-glucose) by intestinal mucosa and muscularis from arterial plasma was studied in order to determine net rate of transport and phosphorylation of the material. The theoretical basis for calculating the rate constants of forward (k 1 x ) and reverse (k 2 x ) transport between plasma and tissue and also phosphorylation (k 3 x ) and dephosphorylation (k 4 x ) of14C labeled deoxy-glucose (DG) was determined. The method can also be used for estimating tissue glucose uptake. The rate constants were found to be:k 1 x =0.669 and 0.873;k 2 x =2.285 and 4.656;k 3 x =0.057 and 0.067;k 4 x =0.091 and 0.097 [s-1] in the mucosa and muscularis, respectively. Glucose utilisation of intestinal mucosa was 2.69 and that of muscularis 2.46 mg/(min×100 g) tissue, respectively. Arterial glucose concentration was constant during the studies, however, it showed a variation from 120 to 250 mg/100 ml of plasma from animal to animal. Tissue glucose uptake or any of the rate constants were not influenced by the plasma level over this range.

KW - Glucose consumption

KW - Glucose transport

KW - Hexokinase

KW - Intestinal mucosa

KW - Muscularis

KW - Phosphatase

UR - http://www.scopus.com/inward/record.url?scp=0021264235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021264235&partnerID=8YFLogxK

U2 - 10.1007/BF00582589

DO - 10.1007/BF00582589

M3 - Article

C2 - 6473075

AN - SCOPUS:0021264235

VL - 401

SP - 233

EP - 238

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 3

ER -