In vivo blockade of thalamic GABA(B) receptors increases excitatory amino-acid levels

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Abstract

The effect of intrathalamic application of GABA(B) receptor antagonists on the basal excitatory amino-acid levels was studied using microdialysis probes implanted in the dorsal lateral geniculate nucleus and in the ventrobasal complex. In both nuclei, continuous perfusion of the GABA(B) receptor antagonist 3-aminopropyl-(diethoxymethyl)-phosphinic acid (CGP 35348) produced an increase in the extracellular concentration of aspartate and (to a lesser extent) glutamate, but no change was observed in the level of taurine, the main amino acid involved in the regulation of brain osmolarity processes. In contrast, 3-amino-2-hydroxy-2-(4-chlorophenyl)-propanesulphonic acid (2-hydroxy-saclofen), another GABA(B) receptor antagonist, failed to affect the extracellular concentration of aspartate, glutamate and taurine. Thus, the basal level of excitatory amino acids in the thalamus in vivo is under the control of CGP 35348-sensitive GABA(B) receptors.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalEuropean Journal of Pharmacology
Volume318
Issue number2-3
DOIs
Publication statusPublished - Dec 30 1996

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GABA-B Receptor Antagonists
GABA-B Receptors
Excitatory Amino Acids
Taurine
Aspartic Acid
Glutamic Acid
Geniculate Bodies
Ventral Thalamic Nuclei
Microdialysis
Thalamus
Osmolar Concentration
Perfusion
Amino Acids
Acids
Brain
CGP 35348

Keywords

  • Aspartate
  • GABA(B) receptor
  • Glutamate
  • Microdialysis
  • Thalamus

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

In vivo blockade of thalamic GABA(B) receptors increases excitatory amino-acid levels. / Nyitrai, G.; Emri, Z.; Crunelli, Vincenzo; Kékesi, K.; Dobolyi, A.; Juhász, G.

In: European Journal of Pharmacology, Vol. 318, No. 2-3, 30.12.1996, p. 295-300.

Research output: Contribution to journalArticle

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