The arterial blood of anaesthetized heparin-treated beagle dogs was directed through a 30 μm diameter pore size screen by a roller pump at a constant rate. As a result, the pressure proximal to the filter continuously increased. The filtration pressure stabilizing concentration of prostacyclin (infused proximal to the filter) was determined. BM 13.177 -similar to PGI2- was able to slow down and stop the increase of filtration pressure (final conc.: 1-10 μM). In addition it could reverse the filter occlusion process. The lowest concentration of BM 13.177 (0.1-1 μM) did not affect significantly the filter occlusion rate, but it markedly enhanced the antiaggregatory effect of PGI2 when these drugs were administered simultaneously. In summary, we can conclude that 1/ generation of thromboxane and endoperoxide plays a key role in the mechanism of the spontaneous platelet aggregation on the filter; 2/ BM 13.177 significantly potentiates the antiaggregatory effect of PGI2 in vivo.
|Journal||Biomedica Biochimica Acta|
|Publication status||Published - Dec 1 1988|
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