In vivo and in vitro induction of 'tissue' transglutaminase in rat hepatocytes by retinoic acid

M. Piacentini, M. P. Cerù, L. Dini, M. Di Rao, L. Piredda, V. Thomazy, P. J A Davies, L. Fésüs

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Abstract

Tissue transglutaminase (tTG) expression was found to be induced in rat liver following in vivo retinoic acid (RA) treatment (Piacentini et al. (1988) Biochem. J. 253, 33-38). Here we show that the increased enzyme expression in rat liver is at least partially the result of the action of RA in parenchymal cells. In fact, (a) when hepatocytes are isolated from RA-treated animals their transglutaminase protein content is much higher than in similarly isolated control cells; (b) higher tTG protein level is also found by immunoelectronmicroscopy in the hepatocytes of the RA-treated rats as compared with the very low amount detected in the controls; (c) RA induces tTG in hepatocytes under culture conditions as well. One of the functionsof tTG is to form a protein polymer in dying apoptotic cells by ε{lunate}(γ-glutamyl)lysine and, specifically γ-glutamylpolyamine cross-links (Fesus et al. (1989) FEBS Lett. 245, 150-154). Noteworthy, after in vivo and in vitro RA-treatment we could not determine any increase (there was even a slight decrease) in the number of the cross-linked apoptotic envelopes. In keeping with this is the significant reduction of protein bound γ-glutamylpolyamine detected in hepatocytes exposed to RA in culture. These findings suggest that the RA-induced tTG in parenchimal cells is an inactive form.

Original languageEnglish
Pages (from-to)171-179
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1135
Issue number2
DOIs
Publication statusPublished - Jun 10 1992

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Tretinoin
Hepatocytes
Proteins
Transglutaminases
In Vitro Techniques
transglutaminase 2
Liver
Lysine
Polymers
Enzymes

Keywords

  • Apoptosis
  • Cell proliferation
  • Di- and polyamine γ-glutamyl derivative
  • Retinoic acid

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

In vivo and in vitro induction of 'tissue' transglutaminase in rat hepatocytes by retinoic acid. / Piacentini, M.; Cerù, M. P.; Dini, L.; Di Rao, M.; Piredda, L.; Thomazy, V.; Davies, P. J A; Fésüs, L.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1135, No. 2, 10.06.1992, p. 171-179.

Research output: Contribution to journalArticle

Piacentini, M. ; Cerù, M. P. ; Dini, L. ; Di Rao, M. ; Piredda, L. ; Thomazy, V. ; Davies, P. J A ; Fésüs, L. / In vivo and in vitro induction of 'tissue' transglutaminase in rat hepatocytes by retinoic acid. In: Biochimica et Biophysica Acta - Molecular Cell Research. 1992 ; Vol. 1135, No. 2. pp. 171-179.
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