The authors investigated the effect of swine ileal and caecal microbiota on fumonisin B1(FB1) metabolism. In order to determine the absorption and biotransformation of FB1, T-cannula was implanted into the distal part of pigs' ileum and the total quantity of urine and faeces was collected during the feeding of diet containing toxin (45 mg FB1/kg, for 10 days) and thereafter (for 10 days). At the end of the trial several organs, muscle and fat samples were also collected. It could be established that the accumulative absorption of fumonisin B1was 4%. In the chymus, the FB1conversion to aminopentol (AP1) and partially hydrolysed FB1(PHFB1) was 1% and 3.9%, respectively. In the faecal content the main product was PHFB1(47%), with small amount (12%) of AP1. In the examined tissues the efficacy of the FB1conversion to aminopentol and partially hydrolysed FB1was 30% and 20%, respectively. In the in vitro study, suspensions of caecal contents, McDougall buffer and pure FB1(5 μg/ml) were incubated under anaerobic conditions for 0, 12, 24, 48 and 72 hours. In the 48thhours, the conversion of FB1into PHFB1(46%) was nearly equal to the percentile ratio of FB1, while in the 72ndhours it was 49%. In vitro, the conversion of fumonisin B1to AP1was less than 1%. Summarized, the intestinal microflora of pigs is able to transform the intact FB1to a similarly toxic substance (PHFB1) or a more toxic metabolite (AP1).
|Translated title of the contribution||In vivo and in vitro examination of the metabolism of fumonisin B1|
|Number of pages||11|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Jan 1 2007|
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