In vitro translation of mRNA for yeast citrate synthase.

T. Alam, D. Finkelstein, P. A. Srere

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Abstract

The citrate synthase of yeast was purified to homogeneity and shown to have a subunit molecular weight of 52,000. Antibodies were prepared to it in rabbits. Translation of total yeast mRNA using a reticulocyte lysate showed that [3H]leucine was incorporated into a protein precipitable by rabbit anti-yeast citrate synthase with a molecular weight of about 54,000. No incorporation of [35S]methionine into an anti-citrate synthase precipitable protein could be detected in a similar experiment. In vivo labeling of cells with 35SO4 did not result in the labeling of citrate synthase.

Original languageEnglish
Pages (from-to)11181-11185
Number of pages5
JournalJournal of Biological Chemistry
Volume257
Issue number18
Publication statusPublished - Sep 25 1982

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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