In vitro translation-based protein kinase substrate identification

Szilvia K. Nagy, T. Mészáros

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Identification of a particular protein as a physiological substrate towards kinases of interest is an extremely complex process. Under physiological conditions kinases and their putative substrates are in low abundance, and production of active eukaryotic kinases with standard overexpressing methods is an arduous task. Herein, we describe a cell-free in vitro protein translation procedure combined with fluorescent phosphoprotein staining as a simple and rapid method for identification of putative kinase substrates.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-243
Number of pages13
Volume1118
ISBN (Print)9781627037815
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1118
ISSN (Print)10643745

Fingerprint

Protein Kinases
Phosphotransferases
Phosphoproteins
Protein Biosynthesis
Staining and Labeling
In Vitro Techniques
Proteins

Keywords

  • In vitro translation
  • Phosphoprotein staining
  • Phosphorylation assay
  • Protein kinase
  • Substrate identification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nagy, S. K., & Mészáros, T. (2014). In vitro translation-based protein kinase substrate identification. In Methods in Molecular Biology (Vol. 1118, pp. 231-243). (Methods in Molecular Biology; Vol. 1118). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-782-2_15

In vitro translation-based protein kinase substrate identification. / Nagy, Szilvia K.; Mészáros, T.

Methods in Molecular Biology. Vol. 1118 Humana Press Inc., 2014. p. 231-243 (Methods in Molecular Biology; Vol. 1118).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nagy, SK & Mészáros, T 2014, In vitro translation-based protein kinase substrate identification. in Methods in Molecular Biology. vol. 1118, Methods in Molecular Biology, vol. 1118, Humana Press Inc., pp. 231-243. https://doi.org/10.1007/978-1-62703-782-2_15
Nagy SK, Mészáros T. In vitro translation-based protein kinase substrate identification. In Methods in Molecular Biology. Vol. 1118. Humana Press Inc. 2014. p. 231-243. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-782-2_15
Nagy, Szilvia K. ; Mészáros, T. / In vitro translation-based protein kinase substrate identification. Methods in Molecular Biology. Vol. 1118 Humana Press Inc., 2014. pp. 231-243 (Methods in Molecular Biology).
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