In vitro susceptibility of filamentous fungi from mycotic keratitis to azole drugs

C. S. Shobana, A. Mythili, M. Homa, L. Galgóczy, R. Priya, Y. R. Babu Singh, K. Panneerselvam, C. Vágvölgyi, L. Kredics, V. Narendran, P. Manikandan

Research output: Contribution to journalArticle

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Abstract

Objective: The in vitro antifungal activities of azole drugs viz., itraconazole, voriconazole, ketoconazole, econazole and clotrimazole were investigated in order to evaluate their efficacy against filamentous fungi isolated from mycotic keratitis. Methods: The specimen collection was carried out from fungal keratitis patients attending Aravind eye hospital and Post-graduate institute of ophthalmology, Coimbatore, India and was subsequently processed for the isolation of fungi. The dilutions of antifungal drugs were prepared in RPMI 1640 medium. Minimum inhibitory concentrations (MICs) were determined and MIC50 and MIC90 were calculated for each drug tested. Results: A total of 60 fungal isolates were identified as Fusarium spp. (n=30), non-sporulating moulds (n=9), Aspergillus flavus (n=6), Bipolaris spp. (n=6), Exserohilum spp. (n=4), Curvularia spp. (n=3), Alternaria spp. (n=1) and Exophiala spp. (n=1). The MICs of ketoconazole, clotrimazole, voriconazole, econazole and itraconazole for all the fungal isolates ranged between 16μg/mL and 0.03μg/mL, 4μg/mL and 0.015μg/mL, 8μg/mL and 0.015μg/mL, 8μg/mL and 0.015μg/mL and 32μg/mL and 0.06μg/mL respectively. From the MIC50 and MIC90 values, it could be deciphered that in the present study, clotrimazole was more active against the test isolates at lower concentrations (0.12-5μg/mL) when compared to other drugs tested. Conclusion: The results suggest that amongst the tested azole drugs, clotrimazole followed by voriconazole and econazole had lower MICs against moulds isolated from mycotic keratitis.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalJournal de Mycologie Medicale
Volume25
Issue number1
DOIs
Publication statusPublished - Mar 1 2015

Fingerprint

Azoles
Keratitis
Clotrimazole
Econazole
Fungi
Microbial Sensitivity Tests
Ketoconazole
Itraconazole
Pharmaceutical Preparations
Exophiala
Specimen Handling
Aspergillus flavus
Alternaria
Fusarium
Ophthalmology
India
In Vitro Techniques
Voriconazole

Keywords

  • Antifungal susceptibility and azole drugs
  • Fungal isolates
  • Mycotic keratitis

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Shobana, C. S., Mythili, A., Homa, M., Galgóczy, L., Priya, R., Babu Singh, Y. R., ... Manikandan, P. (2015). In vitro susceptibility of filamentous fungi from mycotic keratitis to azole drugs. Journal de Mycologie Medicale, 25(1), 44-49. https://doi.org/10.1016/j.mycmed.2014.10.024

In vitro susceptibility of filamentous fungi from mycotic keratitis to azole drugs. / Shobana, C. S.; Mythili, A.; Homa, M.; Galgóczy, L.; Priya, R.; Babu Singh, Y. R.; Panneerselvam, K.; Vágvölgyi, C.; Kredics, L.; Narendran, V.; Manikandan, P.

In: Journal de Mycologie Medicale, Vol. 25, No. 1, 01.03.2015, p. 44-49.

Research output: Contribution to journalArticle

Shobana, CS, Mythili, A, Homa, M, Galgóczy, L, Priya, R, Babu Singh, YR, Panneerselvam, K, Vágvölgyi, C, Kredics, L, Narendran, V & Manikandan, P 2015, 'In vitro susceptibility of filamentous fungi from mycotic keratitis to azole drugs', Journal de Mycologie Medicale, vol. 25, no. 1, pp. 44-49. https://doi.org/10.1016/j.mycmed.2014.10.024
Shobana, C. S. ; Mythili, A. ; Homa, M. ; Galgóczy, L. ; Priya, R. ; Babu Singh, Y. R. ; Panneerselvam, K. ; Vágvölgyi, C. ; Kredics, L. ; Narendran, V. ; Manikandan, P. / In vitro susceptibility of filamentous fungi from mycotic keratitis to azole drugs. In: Journal de Mycologie Medicale. 2015 ; Vol. 25, No. 1. pp. 44-49.
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AU - Priya, R.

AU - Babu Singh, Y. R.

AU - Panneerselvam, K.

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