In vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compounds

Dieter Rehder, João Costa Pessoa, Carlos F G C Geraldes, M. Margarida C A Castro, Themistoklis Kabanos, T. Kiss, Beate Meier, Giovanni Micera, Lage Pettersson, Maria Rangel, Athanasios Salifoglou, Iztok Turel, Dongren Wang

Research output: Contribution to journalArticle

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Abstract

A representative set of vanadium(IV and V) compounds in varying coordination environments has been tested in the concentration range 1 to 10-6 mM, using transformed mice fibroblasts (cell line SV 3T3), with respect to their short-term cell toxicity (up to 36 hours) and their ability to stimulate glucose uptake by cells. These insulin-mimetic tests have also been carried out with non-transformed human fibroblasts (cell line F26). The compounds under investigation comprise established insulin-mimetic species such as vanadate ([H2VO4]-), [VO(acetylacetonate)2], [VO2(dipicolinate)]- and [VO(maltolate)2], and new systems and coordination compounds containing OO, ON, OS, NS and ONS donor atom sets. A vitality test assay, measuring the reduction equivalents released in the mitochondrial respiratory chain by intracellular glucose degradation, is introduced and the results are counter-checked with 3H-labelled glucose. Most compounds are toxic at the 1 mM concentration level, and most compounds are essentially non-toxic and about as effective as or more potent than insulin at concentrations of 0.01 mM and below. VV compounds tend to be less toxic than VIV compounds, and complexes containing thio functional ligands are somewhat more toxic than others. Generally, ON ligation is superior in insulin-mimetic efficacy to OO or O/NS coordination, irrespective of the vanadium oxidation state. There is, however, no striking correlation between the nature of the ligand systems and the insulin-mimetic potency in these cell culture tests, encompassing 41 vanadium compounds, the results on 22 of which are reported in detail here. The syntheses and characteristics of various new compounds are provided together with selected speciation results. The crystal and molecular structures of {[VO(naph-tris)]2} [where naph-tris is the Schiff base formed between o-hydroxynaphthaldehyde and tris(hydroxymethyl)amine] are reported.

Original languageEnglish
Pages (from-to)384-396
Number of pages13
JournalJournal of Biological Inorganic Chemistry
Volume7
Issue number4-5
DOIs
Publication statusPublished - 2002

Fingerprint

Vanadium
Insulin
Poisons
Fibroblasts
Glucose
Vanadium Compounds
Cells
Ligands
Cell Line
Aptitude
Vanadates
Schiff Bases
Electron Transport
Molecular Structure
Cell culture
Molecular structure
Amines
Ligation
Toxicity
Assays

Keywords

  • Cell culture tests
  • Insulin mimesis
  • Solution speciation
  • Toxicity
  • Vanadium compounds

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Rehder, D., Costa Pessoa, J., Geraldes, C. F. G. C., Castro, M. M. C. A., Kabanos, T., Kiss, T., ... Wang, D. (2002). In vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compounds. Journal of Biological Inorganic Chemistry, 7(4-5), 384-396. https://doi.org/10.1007/s00775-001-0311-5

In vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compounds. / Rehder, Dieter; Costa Pessoa, João; Geraldes, Carlos F G C; Castro, M. Margarida C A; Kabanos, Themistoklis; Kiss, T.; Meier, Beate; Micera, Giovanni; Pettersson, Lage; Rangel, Maria; Salifoglou, Athanasios; Turel, Iztok; Wang, Dongren.

In: Journal of Biological Inorganic Chemistry, Vol. 7, No. 4-5, 2002, p. 384-396.

Research output: Contribution to journalArticle

Rehder, D, Costa Pessoa, J, Geraldes, CFGC, Castro, MMCA, Kabanos, T, Kiss, T, Meier, B, Micera, G, Pettersson, L, Rangel, M, Salifoglou, A, Turel, I & Wang, D 2002, 'In vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compounds', Journal of Biological Inorganic Chemistry, vol. 7, no. 4-5, pp. 384-396. https://doi.org/10.1007/s00775-001-0311-5
Rehder, Dieter ; Costa Pessoa, João ; Geraldes, Carlos F G C ; Castro, M. Margarida C A ; Kabanos, Themistoklis ; Kiss, T. ; Meier, Beate ; Micera, Giovanni ; Pettersson, Lage ; Rangel, Maria ; Salifoglou, Athanasios ; Turel, Iztok ; Wang, Dongren. / In vitro study of the insulin-mimetic behaviour of vanadium(IV, V) coordination compounds. In: Journal of Biological Inorganic Chemistry. 2002 ; Vol. 7, No. 4-5. pp. 384-396.
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