In vitro pollen maturation and successful seed production in detached spikelet cultures in wheat (Triticum aestivum L.)

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Two spring wheat genotypes (cv Orofen and Chinese Spring) were compared for their in vitro pollen maturation capacity in detached spikelet cultures in a defined solid medium. Under these in vitro conditions Chinese Spring produced normal trinucleate pollen in 66.8% and Orofen in only 37.5%. In both cultivars the pollen maturation process from the middle uninucleate stage took approximately 3 days longer in vitro than in vivo. The pollen maturation time depended on the microspore developmental stage at the time that the culturing started. The viability, germination capacity, and fertilizing ability of the in vitro matured pollen also differed between the two genotypes. The seed set achieved in vitro (averagely 12.8%) offers promise for the practical application of this method for producing controlled or selected offspring.

Original languageEnglish
Pages (from-to)286-291
Number of pages6
JournalSexual Plant Reproduction
Issue number4
Publication statusPublished - Nov 1 1992



  • Gametophytic selection
  • In vitro pollen maturation
  • Spikelet culture
  • Triticum aestivum

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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