In vitro neutralization of T‐2 toxin toxicity by a monoclonal antibody

Fiorenza Minervini, Agnes Gyongyosi‐Horvath, Giacomo Lucivero, Angelo Visconti, Lldiko Bama‐Vetro, Lazlo Solti

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2 Citations (Scopus)

Abstract

A T‐2 toxin specific monoclonal antibody, IgGl K, with a low level of ELISA cross‐reactivity to Acetyl T‐2, HT‐2, and iso T‐2 toxins has been produced. The ability of this monoclonal antibocly to neutralize the cytotoxicity of T‐2 toxin in PHA stimulated cultures of human lymphocytes was determined by the MTT method. The complete neutralization of the toxic effect of 0.02 μM T‐2 toxin was obtained with 0.03 μM of MoAb, whereas the 50% neutralizing dose (ND50) was observed at 0.009 of MoAb. Partial neutralization was observed with Acetyl T‐2 toxin (ND, = 0.038 μM) and HT‐2 (ND50 = 0.94 μM). These results could represent a rational for clinical use of T‐2 toxin specific monoclonal antibody in prophylaxis and therapy of T‐2 toxemia. © 1994 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalNatural Toxins
Volume2
Issue number3
DOIs
Publication statusPublished - Jan 1 1994

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Keywords

  • Human Lymphocytes
  • MTT Bioassay
  • Monoclonal Antibody
  • Neutralization of Lymphotoxicity
  • Trichothecene Derivatives
  • T‐2 toxin

ASJC Scopus subject areas

  • Toxicology

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