In vitro model of neurotoxicity of Aβ 1-42 and neuroprotection by a pentapeptide: Irreversible events during the first hour

Zsolt Datki, Rita Papp, Dénes Zádori, Katalin Soós, Lívia Fülöp, Anna Juhász, Gábor Laskay, Csaba Hetényi, Erzsébet Mihalik, Márta Zarándi, Botond Penke

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The cell biology of Alzheimer's disease (AD) is characterized mainly by the neurodegeneration caused by the β-amyloid (Aβ) peptides and by the formation of neurofibrillary tangles. The initial events of neurodegeneration in the brain tissue include synaptic dysfunction and axonopathy. Aβ-induced axonopathy and neurite degeneration were studied in vitro on differentiated human-derived neurotypic SH-SY5Y cells. Different methods were used to investigate the mechanism of action of aggregated Aβ on neuroblastoma cells. Aβ 1-42 aggregated for 1 h induced irreversible changes in the neurite morphology. Change of tau hyperphosphorylation and cell viability (cytoplasmic redox state and active membrane uptake) was irreversible during the first hour after the addition of Aβ 1-42 to the cells. These rapid events indicate that Aβ might induce neurodegeneration even at an early stage of Aβ-cell contact. A novel pentapeptide LPYFD-amide, an analog of Soto's LPFFD, significantly decreased neurite degeneration, tau aggregation, and cell viability reduction induced by Aβ 1-42.

Original languageEnglish
Pages (from-to)507-515
Number of pages9
JournalNeurobiology of Disease
Volume17
Issue number3
DOIs
Publication statusPublished - Dec 1 2004

Keywords

  • Aggregation
  • Neurite degeneration
  • Neuroblastoma cells
  • Neuroprotection
  • β-Amyloid

ASJC Scopus subject areas

  • Neurology

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