In vitro microspore selection in maize anther culture with oxidative-stress stimulators

H. Ambrus, É Darko, L. Szabo, F. Bakos, Z. Király, B. Barnabás

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to produce doubled-haploid maize plants tolerant of oxidative stress, in vitro microspore selection was carried out in anther culture with reactive oxygen species (ROS) progenitors such as paraquat, menadione, tert-butylhydroperoxide (t-BHP), and methionine combined with riboflavin. All the ROS progenitors reduced the anther induction, the formation of microspore-derived structures, and their regeneration potential. Abnormal cell divisions and progeny cell degradation could be observed during the development of microspores treated with ROS progenitors. Menadione and t-BHP influenced the microspore developmental pathway, as menadione induced the formation of embryoids, while t-BHP increased the proportion of calli in the microspore-derived structures. As the result of in vitro selection, 15, 10, 10, and 3 fertile doubled-haploid plants were obtained in cultures treated with paraquat, t-BHP, methionine combined with riboflavin, and menadione, respectively.

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalProtoplasma
Volume228
Issue number1-3
DOIs
Publication statusPublished - Aug 2006

Keywords

  • Anther culture
  • In vitro selection
  • Microspore
  • Oxidative stress
  • Reactive oxygen species progenitor
  • Zea mays

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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