In vitro interaction between fumonisin B1 and the intestinal microflora of pigs

Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, István Bors, András Bonai, Brigitta Bota, Judit Szabóoo-Fodor

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B1 and hydrolysed fumonisin B1 concentration were analysed by liquid chromatograghy - mass spectrometry. There was no significant difference in FB1 concentration between the experimental and the control 2 group (buffer and fumonisin B1) at 0 h incubation, 5.185 ± 0.174 7mu;g/ml compared with 6.433 ± 0.076 μg/ml. Fumonisin B1 concentration in the experimental group was reduced to 4.080 ± 0.065 7mu;g/ml at 24 h and to 2.747 ± 0.548 μg/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B1 was detected after 24 h incubation (0.012 ± 0 μg/ml). At 48 h incubation time, hydrolysed fumonisin B1 concentration was doubled to 0.024 7plsmn; 0.004 μg/ml. These results indicate that fumonisin B1 can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalPolish Journal of Microbiology
Volume66
Issue number2
Publication statusPublished - Jan 1 2017

Fingerprint

Swine
Buffers
Lactobacillus
Bacteria
Control Groups
Prevotella
Gastrointestinal Microbiome
fumonisin B1
In Vitro Techniques
Aerobic Bacteria
Bacteroides
Anaerobic Bacteria
Microbiota
Agar
Mass Spectrometry
Escherichia coli
Polymerase Chain Reaction

Keywords

  • Caecal microbiota
  • Fumonisin B
  • Intestinal microflora pig

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Microbiology (medical)

Cite this

Dang, H. A., Zsolnai, A., Kovacs, M., Bors, I., Bonai, A., Bota, B., & Szabóoo-Fodor, J. (2017). In vitro interaction between fumonisin B1 and the intestinal microflora of pigs. Polish Journal of Microbiology, 66(2), 245-250.

In vitro interaction between fumonisin B1 and the intestinal microflora of pigs. / Dang, Huu Anh; Zsolnai, Attila; Kovacs, Melinda; Bors, István; Bonai, András; Bota, Brigitta; Szabóoo-Fodor, Judit.

In: Polish Journal of Microbiology, Vol. 66, No. 2, 01.01.2017, p. 245-250.

Research output: Contribution to journalArticle

Dang, HA, Zsolnai, A, Kovacs, M, Bors, I, Bonai, A, Bota, B & Szabóoo-Fodor, J 2017, 'In vitro interaction between fumonisin B1 and the intestinal microflora of pigs', Polish Journal of Microbiology, vol. 66, no. 2, pp. 245-250.
Dang, Huu Anh ; Zsolnai, Attila ; Kovacs, Melinda ; Bors, István ; Bonai, András ; Bota, Brigitta ; Szabóoo-Fodor, Judit. / In vitro interaction between fumonisin B1 and the intestinal microflora of pigs. In: Polish Journal of Microbiology. 2017 ; Vol. 66, No. 2. pp. 245-250.
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