In vitro hemorheological effects of parenteral agents used in peripheral arterial disease

Katalin Biro, Barbara Sandor, Andras Toth, Katalin Koltai, Judit Papp, Miklos Rabai, Kalman Toth, Gabor Kesmarky

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Abstract

Peripheral arterial disease (PAD) is a frequent manifestation of systemic atherosclerosis. In PAD hemorheo-logical parameters were defined as risk factors in a number of studies and several therapeutic agents were tried in these conditions. Our study aims to investigate and compare the in vitro hemorheological effects of various drugs generally used in the parenteral treatment of intermittent claudication and critical limb ischemia. Blood samples of healthy male volunteers were incubated with iloprost, alprostadil, pen-toxifylline, sulodexide or pentosan polysulfate at calculated therapeutic serum concentration. Hematocrit (Hct) was determined by microhematocrit centrifuge. Plasma and apparent whole blood viscosities (WBV) were evaluated by capillary viscometer. Red blood cell aggregation was measured by LORCA (laser-assisted optical rotational cell analyzer) aggregometer, and LORCA ektacytometer was used for measuring erythrocyte deformability at 37°C. Iloprost, alprostadil, and pentoxifylline incubation did not have any significant effect on plasma and apparent WBV. Elongation index increased in samples incubated with alprostadil at low shear stresses 0.95 and 0.53 Pa (p < 0.05). Sulodexide significantly improved WBV and Hct/ WBV ratio (p < 0.05). Incubation with pentosan polysulfate resulted in higher WBV, lower Hct/WBV ratio and deterioration in the aggregation parameters (p < 0.05). Sulodexide may have beneficial effect on a macrorheological parameter; alprostadil may improve a microrheological parameter. Hemorheological alterations could be important in PAD patients with hampered vasodilator capacity.

Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalKorea Australia Rheology Journal
Volume26
Issue number2
DOIs
Publication statusPublished - 2014

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Keywords

  • Elongation index
  • In vitro study
  • Peripheral arterial disease
  • Plasma and whole blood viscosity
  • Red blood cell aggregation

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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