In vitro fertilisation of in vivo matured porcine oocytes obtained from prepuberal gilts at different time intervals after hCG injection

J. Rátky, D. Rath, K. P. Brüssow

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The goal of the present study was to find out the best interval after hCG injection in PMSG primed prepuberal gilts for retrieval of in vivo matured oocytes for in vitro fertilisation (IVF). Altogether 66 gilts were superovulated with 1500IU PMSG and 500IU hCG 72 h later. Ovum pick up was performed endoscopically 24, 28, 32 or 36 h after hCG and a total of 869 cumulus-oocyte-complexes (COCs) were aspirated from 1400 follicles. COCs were tested for quality, and an aliquot was immediately fixed and stained to determine meiotic configuration. The remaining COCs were fertilised in vitro using frozen-thawed epididymal semen. Quality and developmental stage of embryos were tested after IVF, and the number of nuclei was counted. At 24 to 32 h after hCG only few oocytes have entered the second meiotic cycle (18 to 25% vs. 58% at 36 h, p

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalActa Veterinaria Hungarica
Volume51
Issue number1
DOIs
Publication statusPublished - 2003

Fingerprint

in vitro fertilization
Fertilization in Vitro
gilts
Oocytes
oocytes
Swine
injection
Injections
swine
Semen
ova
Ovum
semen
embryo (animal)
Embryonic Structures
developmental stages

Keywords

  • In vitro fertilisation
  • In vivo maturation
  • Oocyte
  • Ovum pick up
  • Swine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

In vitro fertilisation of in vivo matured porcine oocytes obtained from prepuberal gilts at different time intervals after hCG injection. / Rátky, J.; Rath, D.; Brüssow, K. P.

In: Acta Veterinaria Hungarica, Vol. 51, No. 1, 2003, p. 95-101.

Research output: Contribution to journalArticle

@article{eded4f64b11a446385d97469ae5dc5c2,
title = "In vitro fertilisation of in vivo matured porcine oocytes obtained from prepuberal gilts at different time intervals after hCG injection",
abstract = "The goal of the present study was to find out the best interval after hCG injection in PMSG primed prepuberal gilts for retrieval of in vivo matured oocytes for in vitro fertilisation (IVF). Altogether 66 gilts were superovulated with 1500IU PMSG and 500IU hCG 72 h later. Ovum pick up was performed endoscopically 24, 28, 32 or 36 h after hCG and a total of 869 cumulus-oocyte-complexes (COCs) were aspirated from 1400 follicles. COCs were tested for quality, and an aliquot was immediately fixed and stained to determine meiotic configuration. The remaining COCs were fertilised in vitro using frozen-thawed epididymal semen. Quality and developmental stage of embryos were tested after IVF, and the number of nuclei was counted. At 24 to 32 h after hCG only few oocytes have entered the second meiotic cycle (18 to 25{\%} vs. 58{\%} at 36 h, p",
keywords = "In vitro fertilisation, In vivo maturation, Oocyte, Ovum pick up, Swine",
author = "J. R{\'a}tky and D. Rath and Br{\"u}ssow, {K. P.}",
year = "2003",
doi = "10.1556/AVet.51.2003.1.9",
language = "English",
volume = "51",
pages = "95--101",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - In vitro fertilisation of in vivo matured porcine oocytes obtained from prepuberal gilts at different time intervals after hCG injection

AU - Rátky, J.

AU - Rath, D.

AU - Brüssow, K. P.

PY - 2003

Y1 - 2003

N2 - The goal of the present study was to find out the best interval after hCG injection in PMSG primed prepuberal gilts for retrieval of in vivo matured oocytes for in vitro fertilisation (IVF). Altogether 66 gilts were superovulated with 1500IU PMSG and 500IU hCG 72 h later. Ovum pick up was performed endoscopically 24, 28, 32 or 36 h after hCG and a total of 869 cumulus-oocyte-complexes (COCs) were aspirated from 1400 follicles. COCs were tested for quality, and an aliquot was immediately fixed and stained to determine meiotic configuration. The remaining COCs were fertilised in vitro using frozen-thawed epididymal semen. Quality and developmental stage of embryos were tested after IVF, and the number of nuclei was counted. At 24 to 32 h after hCG only few oocytes have entered the second meiotic cycle (18 to 25% vs. 58% at 36 h, p

AB - The goal of the present study was to find out the best interval after hCG injection in PMSG primed prepuberal gilts for retrieval of in vivo matured oocytes for in vitro fertilisation (IVF). Altogether 66 gilts were superovulated with 1500IU PMSG and 500IU hCG 72 h later. Ovum pick up was performed endoscopically 24, 28, 32 or 36 h after hCG and a total of 869 cumulus-oocyte-complexes (COCs) were aspirated from 1400 follicles. COCs were tested for quality, and an aliquot was immediately fixed and stained to determine meiotic configuration. The remaining COCs were fertilised in vitro using frozen-thawed epididymal semen. Quality and developmental stage of embryos were tested after IVF, and the number of nuclei was counted. At 24 to 32 h after hCG only few oocytes have entered the second meiotic cycle (18 to 25% vs. 58% at 36 h, p

KW - In vitro fertilisation

KW - In vivo maturation

KW - Oocyte

KW - Ovum pick up

KW - Swine

UR - http://www.scopus.com/inward/record.url?scp=0344837692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344837692&partnerID=8YFLogxK

U2 - 10.1556/AVet.51.2003.1.9

DO - 10.1556/AVet.51.2003.1.9

M3 - Article

C2 - 12688130

AN - SCOPUS:0344837692

VL - 51

SP - 95

EP - 101

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 1

ER -