In vitro efficacy of amphotericin B, 5-fluorocytosine, fluconazole, voriconazole and posaconazole against Candida dubliniensis isolates using time-kill methodology

Original article

Z. Szabo, A. Borbely, G. Kardos, F. Somogyvári, A. Kemény-Beke, L. Asztalos, F. Rozgonyi, L. Majoros

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Candida dubliniensis is a recently described yeast that causes infections in mucosal surfaces as well as sterile body sites. Candida dubliniensis develops resistance to fluconazole (FLC) more rapidly than the closely related species C. albicans. The killing activity of amphotericin B (AMB), 5-fluorocytosine (5FC), FLC, voriconazole (VRC) and posaconazole (POS) was determined against six C. dubliniensis clinical isolates, identified using molecular biological methods and C. dubliniensis CD36 reference strain. Minimum inhibitory concentrations (MICs) were determined using the Clinical and Laboratory Standards Institute standard procedure. Time-kill assays were performed using RPMI-1640 as test media over a 48-h period. AMB proved to be fungicidal at ≥0.5 μg ml-1 against all clinical isolates after 48 h. 5FC was only fungicidal at 32-64× MIC (4-8 μg ml-1) against all C. dubliniensis isolates. FLC, VRC and POS were fungistatic; decrease in colony number was observed only at the highest concentrations tested (8, 4 and 4 μg ml-1, respectively). Triazoles invariably showed fungistatic effect at concentrations attainable in the serum. In clinical situations when a fungicidal antifungal is desirable, AMB may be used.

Original languageEnglish
Pages (from-to)196-199
Number of pages4
JournalMycoses
Volume53
Issue number3
DOIs
Publication statusPublished - May 2010

Fingerprint

Flucytosine
Fluconazole
Amphotericin B
Candida
Microbial Sensitivity Tests
Triazoles
Yeasts
Infection
Serum
Voriconazole
posaconazole
In Vitro Techniques

Keywords

  • Candida dubliniensis
  • Fungicidal
  • Fungistatic
  • Killing curves

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

In vitro efficacy of amphotericin B, 5-fluorocytosine, fluconazole, voriconazole and posaconazole against Candida dubliniensis isolates using time-kill methodology : Original article. / Szabo, Z.; Borbely, A.; Kardos, G.; Somogyvári, F.; Kemény-Beke, A.; Asztalos, L.; Rozgonyi, F.; Majoros, L.

In: Mycoses, Vol. 53, No. 3, 05.2010, p. 196-199.

Research output: Contribution to journalArticle

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