In vitro efficacy of 5 antifungal agents against Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis as determined by time-kill methodology

Zsuzsa Szabó, Judit Szilágyi, Ariana Tavanti, Gábor Kardos, Ferenc Rozgonyi, Sedique Bayegan, László Majoros

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Killing activity of amphotericin B, fluconazole, voriconazole, posaconazole, and 5-fluorocytosine was determined against 6 Candida parapsilosis, 3 Candida orthopsilosis, and 4 Candida metapsilosis clinical isolates. After 24 h, 1 of 6 C. parapsilosis, 1 of 3 C. orthopsilosis, and 3 of 4 C. metapsilosis isolates were killed at 1 to 4 μg/mL (1-8× MIC) amphotericin B. The remaining isolates were killed by 2 to 4 μg/mL amphotericin B after 48 h. Fluconazole was fungistatic at ≥1× MIC (0.5-2 μg/mL) against C. parapsilosis and at ≥2× MIC (4-8 μg/mL) against C. orthopsilosis and C. metapsilosis isolates. Voriconazole inhibited C. parapsilosis at ≥1× MIC (0.015-0.12 μg/mL), but the other 2 species were inhibited only at 4 to 8× MIC (0.25-0.5 μg/mL). Against C. orthopsilosis and C. metapsilosis, posaconazole was fungistatic close to the MIC (0.03-0.06 and 0.015-0.03 μg/mL, respectively). Against C. orthopsilosis and C. metapsilosis, fluconazole and voriconazole, but not posaconazole, seem to be less active in vitro than against C. parapsilosis.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Issue number3
Publication statusPublished - Jul 1 2009



  • "Psilosis group"
  • Amphotericin B
  • Killing curves
  • Triazole antifungals

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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