In-vitro effect of flavonoids from Solidago canadensis extract on glutathione S-transferase

Pál Apáti, Peter J. Houghton, Geoffrey Kite, Glyn B. Steventon, A. Kéry

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Solidago canadensis is typical of a flavonoid-rich herb and the effect of an aqueous ethanol extract on glutathione-S-transferase (GST) activity using HepG2 cells was compared with those of the flavonol quercetin and its glycosides quercitrin and rutin, found as major constituents. The composition of the extract was determined by HPLC and rutin was found to be the major flavonoidal component of the extract. Total GST activity was assessed using 1-chloro-2,4-dinitrobenzene as a substrate. The glycosides rutin and quercitrin gave dose-dependent increases in GST activity, with a 50% and 24.5% increase at 250 mM, respectively, while the aglycone quercetin inhibited the enzyme by 30% at 250 mM. The total extract of the herb gave an overall dose-dependent increase, the fractions corresponding to the flavonoids showed activating effects while those containing caffeic acid derivatives were inhibitory. The activity observed corresponds to that reported for similar compounds invivo using rats, thus the HepG2 cell line could serve as a more satisfactory method of assessing the effects of extracts and compounds on GST.

Original languageEnglish
Pages (from-to)251-256
Number of pages6
JournalJournal of Pharmacy and Pharmacology
Volume58
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Solidago
Glutathione Transferase
Flavonoids
Rutin
Quercetin
Hep G2 Cells
Glycosides
Dinitrochlorobenzene
Ethanol
High Pressure Liquid Chromatography
Cell Line
In Vitro Techniques
Enzymes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

In-vitro effect of flavonoids from Solidago canadensis extract on glutathione S-transferase. / Apáti, Pál; Houghton, Peter J.; Kite, Geoffrey; Steventon, Glyn B.; Kéry, A.

In: Journal of Pharmacy and Pharmacology, Vol. 58, No. 2, 02.2006, p. 251-256.

Research output: Contribution to journalArticle

Apáti, Pál ; Houghton, Peter J. ; Kite, Geoffrey ; Steventon, Glyn B. ; Kéry, A. / In-vitro effect of flavonoids from Solidago canadensis extract on glutathione S-transferase. In: Journal of Pharmacy and Pharmacology. 2006 ; Vol. 58, No. 2. pp. 251-256.
@article{723f2e123e254c6ca8698ae7acf08253,
title = "In-vitro effect of flavonoids from Solidago canadensis extract on glutathione S-transferase",
abstract = "Solidago canadensis is typical of a flavonoid-rich herb and the effect of an aqueous ethanol extract on glutathione-S-transferase (GST) activity using HepG2 cells was compared with those of the flavonol quercetin and its glycosides quercitrin and rutin, found as major constituents. The composition of the extract was determined by HPLC and rutin was found to be the major flavonoidal component of the extract. Total GST activity was assessed using 1-chloro-2,4-dinitrobenzene as a substrate. The glycosides rutin and quercitrin gave dose-dependent increases in GST activity, with a 50{\%} and 24.5{\%} increase at 250 mM, respectively, while the aglycone quercetin inhibited the enzyme by 30{\%} at 250 mM. The total extract of the herb gave an overall dose-dependent increase, the fractions corresponding to the flavonoids showed activating effects while those containing caffeic acid derivatives were inhibitory. The activity observed corresponds to that reported for similar compounds invivo using rats, thus the HepG2 cell line could serve as a more satisfactory method of assessing the effects of extracts and compounds on GST.",
author = "P{\'a}l Ap{\'a}ti and Houghton, {Peter J.} and Geoffrey Kite and Steventon, {Glyn B.} and A. K{\'e}ry",
year = "2006",
month = "2",
doi = "10.1211/jpp.58.2.0013",
language = "English",
volume = "58",
pages = "251--256",
journal = "Journal of Pharmacy and Pharmacology",
issn = "0022-3573",
publisher = "Pharmaceutical Press",
number = "2",

}

TY - JOUR

T1 - In-vitro effect of flavonoids from Solidago canadensis extract on glutathione S-transferase

AU - Apáti, Pál

AU - Houghton, Peter J.

AU - Kite, Geoffrey

AU - Steventon, Glyn B.

AU - Kéry, A.

PY - 2006/2

Y1 - 2006/2

N2 - Solidago canadensis is typical of a flavonoid-rich herb and the effect of an aqueous ethanol extract on glutathione-S-transferase (GST) activity using HepG2 cells was compared with those of the flavonol quercetin and its glycosides quercitrin and rutin, found as major constituents. The composition of the extract was determined by HPLC and rutin was found to be the major flavonoidal component of the extract. Total GST activity was assessed using 1-chloro-2,4-dinitrobenzene as a substrate. The glycosides rutin and quercitrin gave dose-dependent increases in GST activity, with a 50% and 24.5% increase at 250 mM, respectively, while the aglycone quercetin inhibited the enzyme by 30% at 250 mM. The total extract of the herb gave an overall dose-dependent increase, the fractions corresponding to the flavonoids showed activating effects while those containing caffeic acid derivatives were inhibitory. The activity observed corresponds to that reported for similar compounds invivo using rats, thus the HepG2 cell line could serve as a more satisfactory method of assessing the effects of extracts and compounds on GST.

AB - Solidago canadensis is typical of a flavonoid-rich herb and the effect of an aqueous ethanol extract on glutathione-S-transferase (GST) activity using HepG2 cells was compared with those of the flavonol quercetin and its glycosides quercitrin and rutin, found as major constituents. The composition of the extract was determined by HPLC and rutin was found to be the major flavonoidal component of the extract. Total GST activity was assessed using 1-chloro-2,4-dinitrobenzene as a substrate. The glycosides rutin and quercitrin gave dose-dependent increases in GST activity, with a 50% and 24.5% increase at 250 mM, respectively, while the aglycone quercetin inhibited the enzyme by 30% at 250 mM. The total extract of the herb gave an overall dose-dependent increase, the fractions corresponding to the flavonoids showed activating effects while those containing caffeic acid derivatives were inhibitory. The activity observed corresponds to that reported for similar compounds invivo using rats, thus the HepG2 cell line could serve as a more satisfactory method of assessing the effects of extracts and compounds on GST.

UR - http://www.scopus.com/inward/record.url?scp=31544452133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31544452133&partnerID=8YFLogxK

U2 - 10.1211/jpp.58.2.0013

DO - 10.1211/jpp.58.2.0013

M3 - Article

C2 - 16451754

AN - SCOPUS:31544452133

VL - 58

SP - 251

EP - 256

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

SN - 0022-3573

IS - 2

ER -