In vitro distribution of porphyrin metabolites from 10-3 M delta-aminolevulinic acid in primary neural tissue cultures

Irene Durkó, A. Juhász

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The distribution of porphyrin metabolites (uro-7-6-5-copro-3-proto-heme) from 10-3 M delta-aminolevulinic acid (ALA) in neuronal and glial primary cultures was examined by an isotopic technique. In both cell types, 8-9% of the total production (cell + medium) was found in the cells and more than 90% in the medium. In both the cells and the medium the predominant porphyrin fraction was uro + 7 carboxylic. The heme fraction of the cells was also very high. Through the exclusion of ALA-synthetase activity, the "Uroporphyria-like-model" of "hepatic porphyria" was demonstrated.

Original languageEnglish
Pages (from-to)465-468
Number of pages4
JournalNeurochemical Research
Volume12
Issue number5
DOIs
Publication statusPublished - May 1987

Fingerprint

Tissue culture
Aminolevulinic Acid
Porphyrins
Metabolites
Heme
5-Aminolevulinate Synthetase
Hepatic Porphyria
Neuroglia
In Vitro Techniques

Keywords

  • Glia
  • neuronal cells
  • porphyrin metabolites
  • uroporphyria-like-model

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

In vitro distribution of porphyrin metabolites from 10-3 M delta-aminolevulinic acid in primary neural tissue cultures. / Durkó, Irene; Juhász, A.

In: Neurochemical Research, Vol. 12, No. 5, 05.1987, p. 465-468.

Research output: Contribution to journalArticle

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