In vitro development of polyspermic porcine oocytes: Relationship between early fragmentation and excessive number of penetrating spermatozoa

Tamás Somfai, Manabu Ozawa, Junko Noguchi, Hiroyuki Kaneko, Ni Wayan Kurniani Karja, Mokhamad Fahrudin, Michiko Nakai, Naoki Maedomari, A. Dinnyés, Takashi Nagai, Kazuhiro Kikuchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Embryo development during in vitro culture of polyspermic porcine oocytes was investigated in the present study. After in vitro fertilization (IVF) of in vitro matured oocytes, putative zygotes were centrifuged to visualize pronuclei. Two pronuclear (2PN) and poly-pronuclear (PPN) zygotes were selected and cultured in vitro. Their development to the blastocyst stage and total cell numbers, dead cell rates and ploidy at the blastocyst stage and morphology of resultant embryos after first cleavage were compared. A cleavage rate of PPN embryos was lower than that of 2PN (61.3% and 82.2%, respectively), however, the ability of cleaved embryos to develop to the blastocyst stage did not differ between the PPN and the 2PN groups (22.4% and 32.9%, respectively). Also there was no difference in total cell numbers and rates of dead cells between PPN and 2PN blastocysts. The majority of blastocysts in 2PN group were found to be diploid. In contrast, blastocysts in PPN group showed heterogeneous status in their ploidy including polyploidy and mixoploidy, whereas a remarkable proportion (31.3%) of them was found to be diploid. After the first cleavage (at 36 h after IVF), there was no difference in the number of nuclei/embryo between the two groups, nevertheless embryos in PPN group had significantly higher numbers of blastomeres than that of embryos in 2PN group, mainly due to an increased frequency of anuclear blastomeres. The present results indicate that correction of embryo ploidy in polyspermic embryos can occur during IVC. Nevertheless the frequency of partial fragmentation in polyspermic embryos is increased.

Original languageEnglish
Pages (from-to)131-147
Number of pages17
JournalAnimal Reproduction Science
Volume107
Issue number1-2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Oocytes
Spermatozoa
embryo (animal)
oocytes
Swine
Embryonic Structures
spermatozoa
Blastocyst
blastocyst
swine
Ploidies
in vitro fertilization
Fertilization in Vitro
ploidy
Blastomeres
blastomeres
Zygote
zygote
Diploidy
diploidy

Keywords

  • Development
  • Fragmentation
  • Polyspermy
  • Porcine oocyte
  • Pronucleus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Genetics

Cite this

In vitro development of polyspermic porcine oocytes : Relationship between early fragmentation and excessive number of penetrating spermatozoa. / Somfai, Tamás; Ozawa, Manabu; Noguchi, Junko; Kaneko, Hiroyuki; Karja, Ni Wayan Kurniani; Fahrudin, Mokhamad; Nakai, Michiko; Maedomari, Naoki; Dinnyés, A.; Nagai, Takashi; Kikuchi, Kazuhiro.

In: Animal Reproduction Science, Vol. 107, No. 1-2, 08.2008, p. 131-147.

Research output: Contribution to journalArticle

Somfai, T, Ozawa, M, Noguchi, J, Kaneko, H, Karja, NWK, Fahrudin, M, Nakai, M, Maedomari, N, Dinnyés, A, Nagai, T & Kikuchi, K 2008, 'In vitro development of polyspermic porcine oocytes: Relationship between early fragmentation and excessive number of penetrating spermatozoa', Animal Reproduction Science, vol. 107, no. 1-2, pp. 131-147. https://doi.org/10.1016/j.anireprosci.2007.06.025
Somfai, Tamás ; Ozawa, Manabu ; Noguchi, Junko ; Kaneko, Hiroyuki ; Karja, Ni Wayan Kurniani ; Fahrudin, Mokhamad ; Nakai, Michiko ; Maedomari, Naoki ; Dinnyés, A. ; Nagai, Takashi ; Kikuchi, Kazuhiro. / In vitro development of polyspermic porcine oocytes : Relationship between early fragmentation and excessive number of penetrating spermatozoa. In: Animal Reproduction Science. 2008 ; Vol. 107, No. 1-2. pp. 131-147.
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AU - Kaneko, Hiroyuki

AU - Karja, Ni Wayan Kurniani

AU - Fahrudin, Mokhamad

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