In vitro cytotoxic activity of cord blood NK cells against herpes simplex virus type-1 infected purified human term villous cytotrophoblast

M. Zdravkovic, G. Aboagye-Mathiesen, V. Zachar, P. Mosborg-Petersen, F. Tóth, X. Liu, P. Ebbesen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Transplacental infection of the fetus with herpes simplex virus (HSV) is associated with high morbidity. The present study was undertaken to shed light on the possible participation of the fetal immune system in the elimination of HSV from placental unit. In a chromium release assay cultured term villous trophoblast cells, regardless of infection with HSV-1, were found resistant to lysis by cord blood natural killer (CBNK) cells. In contrast to this, CBNK cells exhibited a basal level of cytotoxic activity against placental fibroblasts, which was significantly increased by preceding infection of the target cells with HSV-1. Stimulation of CBNK cells with interferon-P purified from trophoblast (tro-IFN-β) increased the killing of both HSV-1 infected and uninfected fibroblast, while HSV-1-infected and uninfected term villous trophoblast cells remained resistant to lysis. IL-2-stimulated CBNK cells were able to lyse villous trophoblast cells at a low level, but no significant difference in the susceptibility of the HSV-1-infected and uninfected trophoblast cell was detected.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalViral Immunology
Volume7
Issue number3
Publication statusPublished - 1994

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Trophoblasts
Human Herpesvirus 1
Fetal Blood
Natural Killer Cells
Blood Cells
Simplexvirus
Fibroblasts
Infection
Chromium
Interferons
Interleukin-2
Immune System
Fetus
In Vitro Techniques
Morbidity

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Zdravkovic, M., Aboagye-Mathiesen, G., Zachar, V., Mosborg-Petersen, P., Tóth, F., Liu, X., & Ebbesen, P. (1994). In vitro cytotoxic activity of cord blood NK cells against herpes simplex virus type-1 infected purified human term villous cytotrophoblast. Viral Immunology, 7(3), 133-140.

In vitro cytotoxic activity of cord blood NK cells against herpes simplex virus type-1 infected purified human term villous cytotrophoblast. / Zdravkovic, M.; Aboagye-Mathiesen, G.; Zachar, V.; Mosborg-Petersen, P.; Tóth, F.; Liu, X.; Ebbesen, P.

In: Viral Immunology, Vol. 7, No. 3, 1994, p. 133-140.

Research output: Contribution to journalArticle

Zdravkovic, M, Aboagye-Mathiesen, G, Zachar, V, Mosborg-Petersen, P, Tóth, F, Liu, X & Ebbesen, P 1994, 'In vitro cytotoxic activity of cord blood NK cells against herpes simplex virus type-1 infected purified human term villous cytotrophoblast', Viral Immunology, vol. 7, no. 3, pp. 133-140.
Zdravkovic, M. ; Aboagye-Mathiesen, G. ; Zachar, V. ; Mosborg-Petersen, P. ; Tóth, F. ; Liu, X. ; Ebbesen, P. / In vitro cytotoxic activity of cord blood NK cells against herpes simplex virus type-1 infected purified human term villous cytotrophoblast. In: Viral Immunology. 1994 ; Vol. 7, No. 3. pp. 133-140.
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