In vitro comparative effect of Cd2+, Ni2+, and Co2+ on mouse postblastocyst development

Katalin Paksy, Zsolt Forgács, István Gáti

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Postblastocyst development of mouse preembryos was studied in vitro in order to determine direct effect of Cd2+, Ni2+, and Co2+ ions on embryogenesis during the peri-implantation stage. Uterine horns were flushed on Day 4 of pregnancy and expanded blastocysts were cultured for 4 days in the presence of micromolar Cd2+ (1.1-26.4), Ni2+ (0.1-500) or Co2+ (1- 200). Area of trophoblast outgrowth was measured and used as a quantitative toxicological endpoint. Hatching, attachment, outgrowth, and formation of inner cells mass were also registered. Significant adverse effect on the development stages were observed at 2.2μM (Cd2+), at 10μM (Ni2+), and at 100μM (Co2+). Cd2+ and Co2+ decreased the area of trophoblast markedly at concentrations of 1.1 and 10μM, respectively. Ni2+ exposure resulted in a slight increase at 10 μM followed by a marked reduction in the trophoblast area at 250μM. Reduced proliferative ability of trophoblast cells may point to compromised invasiveness of the embryo. The lowest Cd2+ concentration (1.1 μM = 0.25 μg/ml) significantly deteriorating trophoblast development was found to be lower than Cd levels ranging up to 0.512 μg/g, reported in clinical ovarian samples of occupationally nonexposed women. The morphological alteration and loss of cellular contacts in blastocysts induced by exposure to Cd2+, Ni2+, or Co2+ may adversely influence adhesion and recognition events and may disturb aggregation of mononuclear trophoblastic cells to multinucleated cells in the course of periimplantation in vivo as well.

Original languageEnglish
Pages (from-to)340-347
Number of pages8
JournalEnvironmental Research
Issue number4
Publication statusPublished - May 1999



  • Cadmium
  • Cobalt
  • Mouse blastocyst
  • Nickel
  • Trophoblast outgrowth

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Science(all)

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