In vitro and in vivo motility studies of 99mTc HM-PAO labelled sperm cells.

L. Balogh, F. Szász, L. Zöldág, G. Huszenicza, L. Tóth, G. Dabasi, G. Jánoki

Research output: Contribution to journalArticle

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Abstract

The motility of 99mTc HM-PAO radiolabelled sperm cells, labelled as described previously (Balogh et al., 1992) was studied. The active migration of spermatozoa was demonstrated in capillary tubes containing bovine oestrous mucus, using an in vitro motility analyzer. Like nonlabelled sperm cells, the labelled spermatozoa covered a 4-5 cm distance in the capillary tubes during a 10-min run. In vitro motility testing of labelled spermatozoa from different animal species (cattle, rabbit, sheep, horse) did not reveal significant differences. The distribution of spermatozoa within the female genital tract was studied in a previously described animal model (rabbit) and in two new models (sheep, chicken). This method enables the determination and visualization of sperm distribution by a noninvasive technique. The results of in vivo motility tests pave the way for the introduction of a method of unprecedented specificity, which serves for studying the penetrability of the oviduct to spermatozoa.

Original languageEnglish
Pages (from-to)63-77
Number of pages15
JournalActa Veterinaria Hungarica
Volume43
Issue number1
Publication statusPublished - 1995

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Spermatozoa
spermatozoa
cells
Sheep
rabbits
Rabbits
sheep
Oviducts
In Vitro Techniques
cattle
female genitalia
Mucus
oviducts
mucus
Horses
Chickens
Animal Models
animal models
methodology
testing

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Balogh, L., Szász, F., Zöldág, L., Huszenicza, G., Tóth, L., Dabasi, G., & Jánoki, G. (1995). In vitro and in vivo motility studies of 99mTc HM-PAO labelled sperm cells. Acta Veterinaria Hungarica, 43(1), 63-77.

In vitro and in vivo motility studies of 99mTc HM-PAO labelled sperm cells. / Balogh, L.; Szász, F.; Zöldág, L.; Huszenicza, G.; Tóth, L.; Dabasi, G.; Jánoki, G.

In: Acta Veterinaria Hungarica, Vol. 43, No. 1, 1995, p. 63-77.

Research output: Contribution to journalArticle

Balogh, L, Szász, F, Zöldág, L, Huszenicza, G, Tóth, L, Dabasi, G & Jánoki, G 1995, 'In vitro and in vivo motility studies of 99mTc HM-PAO labelled sperm cells.', Acta Veterinaria Hungarica, vol. 43, no. 1, pp. 63-77.
Balogh, L. ; Szász, F. ; Zöldág, L. ; Huszenicza, G. ; Tóth, L. ; Dabasi, G. ; Jánoki, G. / In vitro and in vivo motility studies of 99mTc HM-PAO labelled sperm cells. In: Acta Veterinaria Hungarica. 1995 ; Vol. 43, No. 1. pp. 63-77.
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