In vitro and in vivo effects of formamidines in locust (Locusta migratoria migratorioides)

L. Hiripi, L. Nagy, R. M. Hollingworth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In vivo and in vitro experiments were used to study the effects of formamidines in the locust, Locusta migratoria migratorioides. In vivo the lethal and the antifeeding effects, in vitro the inhibition of the binding of a selective 3H-ligand to the receptors of octopamine, tyramine, dopamine, serotonin and γ-amino butiric acid were studied. We have demonstrated that demethylchlordimeform is specific agonist to octopamine receptor, having high affinity to octopamine receptor, a moderate affinity to tyramine receptor and a low affinity to dopamine, serotonin and to γ-amino butiric acid receptors. The demethylated chlordimeform analogoues, demethylchlordimeform and didemethylchlordimeform have higher affinity to the octopamine receptor than the parent compound. The formamidines had a toxic and an antifeeding effects when injected into the locust. The half lethal doses (LD50) and the feeding inhibition were correlated with the affinity of the compounds (K(i)). The ring substitutions of the molecule have alterated the both affinity and in vivo effect of the compounds. The most effective ring substitution pattern is 2,4-disubstitution with a combination of methyl groups or halogens. Our results suggest that the lethal effect of formamidines is mediated through the octopamine receptor.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalActa Biologica Hungarica
Volume50
Issue number1-3
Publication statusPublished - 1999

Fingerprint

Locusta migratoria migratorioides
Locusta migratoria
Grasshoppers
locust
octopamine
locusts
receptors
antifeeding activity
tyramine
Chlorphenamidine
Dopamine
dopamine
Serotonin
serotonin
substitution
Substitution reactions
amino acid
Amino Acid Receptors
chlordimeform
Tyramine

Keywords

  • Formamidine
  • Insect Locusta migratoria migratorioides
  • Octopamine
  • Receptor
  • Toxicity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

In vitro and in vivo effects of formamidines in locust (Locusta migratoria migratorioides). / Hiripi, L.; Nagy, L.; Hollingworth, R. M.

In: Acta Biologica Hungarica, Vol. 50, No. 1-3, 1999, p. 81-87.

Research output: Contribution to journalArticle

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