In vitro and in vivo antitumor effect of 2-methoxyestradiol on human melanoma

Judit Dobos, József Tímár, József Bocsi, Zsuzsanna Burián, Katalin Nagy, Gábor Barna, István Peták, Andrea Ladányi

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46 Citations (Scopus)


2-methoxyestradiol (2ME2) is an endogenous metabolite of estradiol with estrogen-receptor-independent antitumor and antiangiogenic activity. We examined the effects of 2ME2 on the cellular proliferation of 8 human melanoma cell lines. We show that 2ME2 inhibited cell proliferation by inducing apoptosis and an arrest in the G 2/M phase, and the mechanism of action involved microtubules, mitochondrial damage and caspase activation. In male SCID mice, 2ME2 was effective in reducing primary tumor weight and the number of liver metastases after intrasplenic injection of human melanoma cells. In the metastases, we found a significantly higher rate of apoptotic cells, after 2ME2 treatment. These findings on the antitumor effect of 2ME 2 in cell culture as well as in an animal model may have implications for designing alternative treatment options for patients with advanced malignant melanoma.

Original languageEnglish
Pages (from-to)771-776
Number of pages6
JournalInternational Journal of Cancer
Issue number5
Publication statusPublished - Dec 10 2004


  • 2-methoxyestradiol
  • Apoptosis
  • Melanoma
  • Metastasis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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