In vitro and in vivo antitumor effect of 2-methoxyestradiol on human melanoma

J. Dobos, J. Tímár, József Bocsi, Zsuzsanna Burián, Katalin Nagy, G. Barna, I. Peták, A. Ladányi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

2-methoxyestradiol (2ME2) is an endogenous metabolite of estradiol with estrogen-receptor-independent antitumor and antiangiogenic activity. We examined the effects of 2ME2 on the cellular proliferation of 8 human melanoma cell lines. We show that 2ME2 inhibited cell proliferation by inducing apoptosis and an arrest in the G 2/M phase, and the mechanism of action involved microtubules, mitochondrial damage and caspase activation. In male SCID mice, 2ME2 was effective in reducing primary tumor weight and the number of liver metastases after intrasplenic injection of human melanoma cells. In the metastases, we found a significantly higher rate of apoptotic cells, after 2ME2 treatment. These findings on the antitumor effect of 2ME 2 in cell culture as well as in an animal model may have implications for designing alternative treatment options for patients with advanced malignant melanoma.

Original languageEnglish
Pages (from-to)771-776
Number of pages6
JournalInternational Journal of Cancer
Volume112
Issue number5
DOIs
Publication statusPublished - Dec 10 2004

Fingerprint

Melanoma
Cell Proliferation
Neoplasm Metastasis
SCID Mice
Caspases
Tumor Burden
Microtubules
Estrogen Receptors
Cell Division
Estradiol
Animal Models
Cell Culture Techniques
Apoptosis
Cell Line
Injections
Liver
Therapeutics
In Vitro Techniques
2-methoxyestradiol

Keywords

  • 2-methoxyestradiol
  • Apoptosis
  • Melanoma
  • Metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro and in vivo antitumor effect of 2-methoxyestradiol on human melanoma. / Dobos, J.; Tímár, J.; Bocsi, József; Burián, Zsuzsanna; Nagy, Katalin; Barna, G.; Peták, I.; Ladányi, A.

In: International Journal of Cancer, Vol. 112, No. 5, 10.12.2004, p. 771-776.

Research output: Contribution to journalArticle

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AB - 2-methoxyestradiol (2ME2) is an endogenous metabolite of estradiol with estrogen-receptor-independent antitumor and antiangiogenic activity. We examined the effects of 2ME2 on the cellular proliferation of 8 human melanoma cell lines. We show that 2ME2 inhibited cell proliferation by inducing apoptosis and an arrest in the G 2/M phase, and the mechanism of action involved microtubules, mitochondrial damage and caspase activation. In male SCID mice, 2ME2 was effective in reducing primary tumor weight and the number of liver metastases after intrasplenic injection of human melanoma cells. In the metastases, we found a significantly higher rate of apoptotic cells, after 2ME2 treatment. These findings on the antitumor effect of 2ME 2 in cell culture as well as in an animal model may have implications for designing alternative treatment options for patients with advanced malignant melanoma.

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