In Vitro analysis of predicted DNA-binding sites for the Stl repressor of the Staphylococcus aureus SaPIBov1 pathogenicity island

Veronika Papp-Kádár, Judit Eszter Szabó, Kinga Nyíri, B. Vértessy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The regulation model of the Staphylococcus aureus pathogenicity island SaPIbov1 transfer was recently reported. The repressor protein Stl obstructs the expression of SaPI proteins Str and Xis, latter which is responsible for mobilization initiation. Upon φ11 phage infection of S. aureus. phage dUTPase activates the SaPI transfer via Stl-dUTPase complex formation. Our aim was to predict the binding sites for the Stl repressor within the S. aureus pathogenicity island DNA sequence. We found that Stl was capable to bind to three 23-mer oligonucleotides, two of those constituting sequence segments in the stl-str, while the other corresponding to sequence segment within the str-xis intergenic region. Within these oligonucleotides, mutational analysis revealed that the predicted binding site for the Stl protein exists as a palindromic segment in both intergenic locations. The palindromes are built as 6-mer repeat sequences involved in Stl binding. The 6-mer repeats are separated by a 5 oligonucleotides long, nonspecific sequence. Future examination of the interaction between Stl and its binding sites in vivo will provide a molecular explanation for the mechanisms of gene repression and gene activation exerted simultaneously by the Stl protein in regulating transfer of the SaPIbov1 pathogenicity island in S. aureus.

Original languageEnglish
Article numbere0158793
JournalPLoS One
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

pathogenicity islands
Genomic Islands
Oligonucleotides
Staphylococcus aureus
binding sites
Bacteriophages
oligonucleotides
Binding Sites
DNA
Genes
bacteriophages
Repressor Proteins
Proteins
Intergenic DNA
DNA sequences
gene activation
Chemical activation
intergenic DNA
Transcriptional Activation
proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

In Vitro analysis of predicted DNA-binding sites for the Stl repressor of the Staphylococcus aureus SaPIBov1 pathogenicity island. / Papp-Kádár, Veronika; Szabó, Judit Eszter; Nyíri, Kinga; Vértessy, B.

In: PLoS One, Vol. 11, No. 7, e0158793, 01.07.2016.

Research output: Contribution to journalArticle

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