In vitro activity of Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole against different dermatophytes

László Galgöczy, T. Papp, I. Pócsi, Nikoletta Hegedñs, C. Vágvölgyi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 μg ml-1 when it was used alone, or at constant concentration (100 μg ml-1) in combination with FCZ at from 0.25 to 32 μg ml-1. The MICs for FCZ were found to be between 0.25 and 128 μg ml-1. PAF caused a fungicidal effect at 200 μg ml-1 and reduced growth at between 50 and 200 μg ml-1. Total growth inhibition with fungistatic activity was detected at 64 μg ml-1 of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 μg ml-1 FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.

Original languageEnglish
Pages (from-to)463-470
Number of pages8
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume94
Issue number3
DOIs
Publication statusPublished - 2008

Fingerprint

Arthrodermataceae
Fluconazole
Trichophyton
Microsporum
Growth
Germination
Penicillium chrysogenum PAF protein
In Vitro Techniques
Fungi

Keywords

  • Drug interaction
  • Fluconazole
  • Microsporum
  • PAF
  • Trichophyton

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

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abstract = "Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 μg ml-1 when it was used alone, or at constant concentration (100 μg ml-1) in combination with FCZ at from 0.25 to 32 μg ml-1. The MICs for FCZ were found to be between 0.25 and 128 μg ml-1. PAF caused a fungicidal effect at 200 μg ml-1 and reduced growth at between 50 and 200 μg ml-1. Total growth inhibition with fungistatic activity was detected at 64 μg ml-1 of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 μg ml-1 FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.",
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AU - Pócsi, I.

AU - Hegedñs, Nikoletta

AU - Vágvölgyi, C.

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