Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 μg ml-1 when it was used alone, or at constant concentration (100 μg ml-1) in combination with FCZ at from 0.25 to 32 μg ml-1. The MICs for FCZ were found to be between 0.25 and 128 μg ml-1. PAF caused a fungicidal effect at 200 μg ml-1 and reduced growth at between 50 and 200 μg ml-1. Total growth inhibition with fungistatic activity was detected at 64 μg ml-1 of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 μg ml-1 FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.
|Number of pages||8|
|Journal||Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology|
|Publication status||Published - Jan 1 2008|
- Drug interaction
ASJC Scopus subject areas
- Molecular Biology