In the fast lane: Large-scale bacterial genome engineering

Tamás Fehér, Valerie Burland, G. Pósfai

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The last few years have witnessed rapid progress in bacterial genome engineering. The long-established, standard ways of DNA synthesis, modification, transfer into living cells, and incorporation into genomes have given way to more effective, large-scale, robust genome modification protocols. Expansion of these engineering capabilities is due to several factors. Key advances include: (i) progress in oligonucleotide synthesis and in vitro and in vivo assembly methods, (ii) optimization of recombineering techniques, (iii) introduction of parallel, large-scale, combinatorial, and automated genome modification procedures, and (iv) rapid identification of the modifications by barcode-based analysis and sequencing. Combination of the brute force of these techniques with sophisticated bioinformatic design and modeling opens up new avenues for the analysis of gene functions and cellular network interactions, but also in engineering more effective producer strains. This review presents a summary of recent technological advances in bacterial genome engineering.

Original languageEnglish
Pages (from-to)72-79
Number of pages8
JournalJournal of Biotechnology
Volume160
Issue number1-2
DOIs
Publication statusPublished - Jul 31 2012

Fingerprint

Bacterial Genomes
Genes
Genome
Computational Biology
Oligonucleotides
Bioinformatics
DNA
Cells

Keywords

  • Bacteria
  • Genome engineering
  • High throughput
  • Synthetic biology

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

In the fast lane : Large-scale bacterial genome engineering. / Fehér, Tamás; Burland, Valerie; Pósfai, G.

In: Journal of Biotechnology, Vol. 160, No. 1-2, 31.07.2012, p. 72-79.

Research output: Contribution to journalArticle

Fehér, Tamás ; Burland, Valerie ; Pósfai, G. / In the fast lane : Large-scale bacterial genome engineering. In: Journal of Biotechnology. 2012 ; Vol. 160, No. 1-2. pp. 72-79.
@article{8d285649bc5341659eb263aeda743557,
title = "In the fast lane: Large-scale bacterial genome engineering",
abstract = "The last few years have witnessed rapid progress in bacterial genome engineering. The long-established, standard ways of DNA synthesis, modification, transfer into living cells, and incorporation into genomes have given way to more effective, large-scale, robust genome modification protocols. Expansion of these engineering capabilities is due to several factors. Key advances include: (i) progress in oligonucleotide synthesis and in vitro and in vivo assembly methods, (ii) optimization of recombineering techniques, (iii) introduction of parallel, large-scale, combinatorial, and automated genome modification procedures, and (iv) rapid identification of the modifications by barcode-based analysis and sequencing. Combination of the brute force of these techniques with sophisticated bioinformatic design and modeling opens up new avenues for the analysis of gene functions and cellular network interactions, but also in engineering more effective producer strains. This review presents a summary of recent technological advances in bacterial genome engineering.",
keywords = "Bacteria, Genome engineering, High throughput, Synthetic biology",
author = "Tam{\'a}s Feh{\'e}r and Valerie Burland and G. P{\'o}sfai",
year = "2012",
month = "7",
day = "31",
doi = "10.1016/j.jbiotec.2012.02.012",
language = "English",
volume = "160",
pages = "72--79",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - In the fast lane

T2 - Large-scale bacterial genome engineering

AU - Fehér, Tamás

AU - Burland, Valerie

AU - Pósfai, G.

PY - 2012/7/31

Y1 - 2012/7/31

N2 - The last few years have witnessed rapid progress in bacterial genome engineering. The long-established, standard ways of DNA synthesis, modification, transfer into living cells, and incorporation into genomes have given way to more effective, large-scale, robust genome modification protocols. Expansion of these engineering capabilities is due to several factors. Key advances include: (i) progress in oligonucleotide synthesis and in vitro and in vivo assembly methods, (ii) optimization of recombineering techniques, (iii) introduction of parallel, large-scale, combinatorial, and automated genome modification procedures, and (iv) rapid identification of the modifications by barcode-based analysis and sequencing. Combination of the brute force of these techniques with sophisticated bioinformatic design and modeling opens up new avenues for the analysis of gene functions and cellular network interactions, but also in engineering more effective producer strains. This review presents a summary of recent technological advances in bacterial genome engineering.

AB - The last few years have witnessed rapid progress in bacterial genome engineering. The long-established, standard ways of DNA synthesis, modification, transfer into living cells, and incorporation into genomes have given way to more effective, large-scale, robust genome modification protocols. Expansion of these engineering capabilities is due to several factors. Key advances include: (i) progress in oligonucleotide synthesis and in vitro and in vivo assembly methods, (ii) optimization of recombineering techniques, (iii) introduction of parallel, large-scale, combinatorial, and automated genome modification procedures, and (iv) rapid identification of the modifications by barcode-based analysis and sequencing. Combination of the brute force of these techniques with sophisticated bioinformatic design and modeling opens up new avenues for the analysis of gene functions and cellular network interactions, but also in engineering more effective producer strains. This review presents a summary of recent technological advances in bacterial genome engineering.

KW - Bacteria

KW - Genome engineering

KW - High throughput

KW - Synthetic biology

UR - http://www.scopus.com/inward/record.url?scp=84862239359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862239359&partnerID=8YFLogxK

U2 - 10.1016/j.jbiotec.2012.02.012

DO - 10.1016/j.jbiotec.2012.02.012

M3 - Article

C2 - 22406111

AN - SCOPUS:84862239359

VL - 160

SP - 72

EP - 79

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1-2

ER -