In situ tissue engineering: Endothelial growth patterns as a function of flow diverter design

Miklos Marosfoi, Erin T. Langan, Lara Strittmatter, Kajo Van Der Marel, Srinivasan Vedantham, Jennifer Arends, Ivan R. Lylyk, Siddharth Loganathan, Gregory M. Hendricks, I. Szikora, Ajit S. Puri, Ajay K. Wakhloo, Matthew J. Gounis

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background Vascular remodeling in response to implantation of a tissue engineering scaffold such as a flow diverter (FD) leads to the cure of intracranial aneurysms. We hypothesize that the vascular response is dependent on FD design, and CD34+ progenitor cells play an important role in the endothelialization of the implant. Methods Sixteen rabbit aneurysms were randomly treated with two different single-layer braided FDs made of cobalt.chrome alloys. The FD-48 and FD-72 devices had 48 and 72 wires, respectively. Aneurysm occlusion rate was assessed during the final digital subtraction angiogram at 10, 20, 30, and 60 days (n=2 per device per time point). Implanted vessels were analyzed with scanning electron microscopy for tissue coverage, endothelialization, and immuno-gold labeling for CD34+ cells. Results Complete aneurysm occlusion rates were similar between the devices; however, complete or near complete occlusion was more frequently observed in aneurysms with neck ≤4.2 mm (p=0.008). Total tissue coverage at 10 days over the surface of the FD-48 and FD-72 devices was 56.4±11.6% and 76.6±3.6%, respectively. Endothelial cell growth over the surface was time-dependent for the FD-72 device (Spearmanfs r=0.86, p=0.013) but not for the FD-48 device (Spearmanfs r=.0.59, p=0.094). The endothelialization score was marginally correlated with the distance from the aneurysm neck for the FD-48 device (Spearmanfs r=1, p=0.083) but not for the FD-72 device (Spearmanfs r=0.8, p=0.33). CD34+ cells were present along the entirety of both devices at all time points. Conclusions This study gives preliminary evidence that temporal and spatial endothelialization is dependent on FD design. Circulating CD34+ progenitor cells contribute to endothelialization throughout the healing process.

Original languageEnglish
Pages (from-to)994-998
Number of pages5
JournalJournal of NeuroInterventional Surgery
Volume9
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

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Tissue Engineering
Equipment and Supplies
Growth
Aneurysm
Stem Cells
Tissue Scaffolds
Intracranial Aneurysm
Cobalt
Gold
Electron Scanning Microscopy
Blood Vessels
Angiography
Neck
Endothelial Cells
Rabbits

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Cite this

Marosfoi, M., Langan, E. T., Strittmatter, L., Van Der Marel, K., Vedantham, S., Arends, J., ... Gounis, M. J. (2017). In situ tissue engineering: Endothelial growth patterns as a function of flow diverter design. Journal of NeuroInterventional Surgery, 9(10), 994-998. https://doi.org/10.1136/neurintsurg-2016-012669

In situ tissue engineering : Endothelial growth patterns as a function of flow diverter design. / Marosfoi, Miklos; Langan, Erin T.; Strittmatter, Lara; Van Der Marel, Kajo; Vedantham, Srinivasan; Arends, Jennifer; Lylyk, Ivan R.; Loganathan, Siddharth; Hendricks, Gregory M.; Szikora, I.; Puri, Ajit S.; Wakhloo, Ajay K.; Gounis, Matthew J.

In: Journal of NeuroInterventional Surgery, Vol. 9, No. 10, 01.10.2017, p. 994-998.

Research output: Contribution to journalArticle

Marosfoi, M, Langan, ET, Strittmatter, L, Van Der Marel, K, Vedantham, S, Arends, J, Lylyk, IR, Loganathan, S, Hendricks, GM, Szikora, I, Puri, AS, Wakhloo, AK & Gounis, MJ 2017, 'In situ tissue engineering: Endothelial growth patterns as a function of flow diverter design', Journal of NeuroInterventional Surgery, vol. 9, no. 10, pp. 994-998. https://doi.org/10.1136/neurintsurg-2016-012669
Marosfoi, Miklos ; Langan, Erin T. ; Strittmatter, Lara ; Van Der Marel, Kajo ; Vedantham, Srinivasan ; Arends, Jennifer ; Lylyk, Ivan R. ; Loganathan, Siddharth ; Hendricks, Gregory M. ; Szikora, I. ; Puri, Ajit S. ; Wakhloo, Ajay K. ; Gounis, Matthew J. / In situ tissue engineering : Endothelial growth patterns as a function of flow diverter design. In: Journal of NeuroInterventional Surgery. 2017 ; Vol. 9, No. 10. pp. 994-998.
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abstract = "Background Vascular remodeling in response to implantation of a tissue engineering scaffold such as a flow diverter (FD) leads to the cure of intracranial aneurysms. We hypothesize that the vascular response is dependent on FD design, and CD34+ progenitor cells play an important role in the endothelialization of the implant. Methods Sixteen rabbit aneurysms were randomly treated with two different single-layer braided FDs made of cobalt.chrome alloys. The FD-48 and FD-72 devices had 48 and 72 wires, respectively. Aneurysm occlusion rate was assessed during the final digital subtraction angiogram at 10, 20, 30, and 60 days (n=2 per device per time point). Implanted vessels were analyzed with scanning electron microscopy for tissue coverage, endothelialization, and immuno-gold labeling for CD34+ cells. Results Complete aneurysm occlusion rates were similar between the devices; however, complete or near complete occlusion was more frequently observed in aneurysms with neck ≤4.2 mm (p=0.008). Total tissue coverage at 10 days over the surface of the FD-48 and FD-72 devices was 56.4±11.6{\%} and 76.6±3.6{\%}, respectively. Endothelial cell growth over the surface was time-dependent for the FD-72 device (Spearmanfs r=0.86, p=0.013) but not for the FD-48 device (Spearmanfs r=.0.59, p=0.094). The endothelialization score was marginally correlated with the distance from the aneurysm neck for the FD-48 device (Spearmanfs r=1, p=0.083) but not for the FD-72 device (Spearmanfs r=0.8, p=0.33). CD34+ cells were present along the entirety of both devices at all time points. Conclusions This study gives preliminary evidence that temporal and spatial endothelialization is dependent on FD design. Circulating CD34+ progenitor cells contribute to endothelialization throughout the healing process.",
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AU - Strittmatter, Lara

AU - Van Der Marel, Kajo

AU - Vedantham, Srinivasan

AU - Arends, Jennifer

AU - Lylyk, Ivan R.

AU - Loganathan, Siddharth

AU - Hendricks, Gregory M.

AU - Szikora, I.

AU - Puri, Ajit S.

AU - Wakhloo, Ajay K.

AU - Gounis, Matthew J.

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N2 - Background Vascular remodeling in response to implantation of a tissue engineering scaffold such as a flow diverter (FD) leads to the cure of intracranial aneurysms. We hypothesize that the vascular response is dependent on FD design, and CD34+ progenitor cells play an important role in the endothelialization of the implant. Methods Sixteen rabbit aneurysms were randomly treated with two different single-layer braided FDs made of cobalt.chrome alloys. The FD-48 and FD-72 devices had 48 and 72 wires, respectively. Aneurysm occlusion rate was assessed during the final digital subtraction angiogram at 10, 20, 30, and 60 days (n=2 per device per time point). Implanted vessels were analyzed with scanning electron microscopy for tissue coverage, endothelialization, and immuno-gold labeling for CD34+ cells. Results Complete aneurysm occlusion rates were similar between the devices; however, complete or near complete occlusion was more frequently observed in aneurysms with neck ≤4.2 mm (p=0.008). Total tissue coverage at 10 days over the surface of the FD-48 and FD-72 devices was 56.4±11.6% and 76.6±3.6%, respectively. Endothelial cell growth over the surface was time-dependent for the FD-72 device (Spearmanfs r=0.86, p=0.013) but not for the FD-48 device (Spearmanfs r=.0.59, p=0.094). The endothelialization score was marginally correlated with the distance from the aneurysm neck for the FD-48 device (Spearmanfs r=1, p=0.083) but not for the FD-72 device (Spearmanfs r=0.8, p=0.33). CD34+ cells were present along the entirety of both devices at all time points. Conclusions This study gives preliminary evidence that temporal and spatial endothelialization is dependent on FD design. Circulating CD34+ progenitor cells contribute to endothelialization throughout the healing process.

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