In situ spectroscopic ellipsometry study of protein immobilization on different substrates using liquid cells

Andrea Nemeth, Peter Kozma, Timea Hülber, Sándor Kurunczi, Robert Horvath, Peter Petrik, Adél Muskotál, Ferenc Vonderviszt, Csaba Hõs, Miklós Fried, József Gyulai, István Bársony

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The influence of substrate materials on protein adsorption was studied by spectroscopic ellipsometry (SE) and atomic force microscopy. For model proteins fibrinogen and flagellar filaments were chosen and their kinetics of adsorption, surface coverage and adsorbed amount on virgin and chemically activated SiO2 and Ta2O5 thin films were investigated. In case of flagellar filaments the SE data were analyzed with an effective medium model that accounted for the vertical density distribution of the adsorbed protein layer. Adsorption was measured in situ using flow cells with various fluid volume. Compared to commercially available cells, a flow cell with significantly smaller volume was constructed for cost-effective measurements. The development of the flow cell was supported by finite element fluid dynamics calculations.

Original languageEnglish
Pages (from-to)730-735
Number of pages6
JournalSensor Letters
Volume8
Issue number5
DOIs
Publication statusPublished - Oct 1 2010

Keywords

  • Biosensor
  • Liquid cell
  • Protein adsorption
  • Spectroscopic ellipsometry

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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