In situ localization of manganese peroxidase production in mycelial pallets of Phanerochaete chrysosporium

G. Jiménez-Tobon, W. Kurzatkowski, B. Rozbicka, J. Solecka, I. Pócsi, M. J. Penninckx

Research output: Contribution to journalArticle

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Abstract

The ultrastructure of Phanerochaete chrysosporium hyphae from pellets in submerged liquid cultures was investigated in order to learn more about the interrelation between fungal architecture and manganese peroxidase (MnP) production. At day 2 of cultivation, some subapical regions of hyphae in the outer and middle zones of the pellet initiated differentiation into intercalary thick-walled chlamydospore-like cells of about 10 μm diameter. At the periphery of the cytoplasm of these cells, a large number of mitochondria and Golgi-like vesicles were observed. The sites of MnP production were localized at different stages of cultivation by an immunolabelling procedure. The immunomarker of MnP was mainly concentrated in the chlamydospore-like cells and principally distributed in Golgi-like vesicles located at the periphery of the cytoplasm. The apices of hyphae in the outer layer of the pellets were apparently minor sites of MnP production. Maximal MnP release into the culture supernatant coincided with apparent autolysis of the chlamydospore-like cells. Production of extracellular autolytic chitinase and protease coincided with the disappearance of these structures from the pellets. The chlamydospore-like cells observed in the mycelial pellets of P. chrysosporium could be metabolically active entities operating as an enzyme reservoir, delivering their content into the surrounding medium possibly by an enzyme-mediated autolytic process.

Original languageEnglish
Pages (from-to)3121-3127
Number of pages7
JournalMicrobiology
Volume149
Issue number11
Publication statusPublished - Nov 2003

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manganese peroxidase
Phanerochaete
Hyphae
Cytoplasm
Autolysis
Chitinases
Enzymes
Mitochondria
Peptide Hydrolases

ASJC Scopus subject areas

  • Microbiology

Cite this

Jiménez-Tobon, G., Kurzatkowski, W., Rozbicka, B., Solecka, J., Pócsi, I., & Penninckx, M. J. (2003). In situ localization of manganese peroxidase production in mycelial pallets of Phanerochaete chrysosporium. Microbiology, 149(11), 3121-3127.

In situ localization of manganese peroxidase production in mycelial pallets of Phanerochaete chrysosporium. / Jiménez-Tobon, G.; Kurzatkowski, W.; Rozbicka, B.; Solecka, J.; Pócsi, I.; Penninckx, M. J.

In: Microbiology, Vol. 149, No. 11, 11.2003, p. 3121-3127.

Research output: Contribution to journalArticle

Jiménez-Tobon, G, Kurzatkowski, W, Rozbicka, B, Solecka, J, Pócsi, I & Penninckx, MJ 2003, 'In situ localization of manganese peroxidase production in mycelial pallets of Phanerochaete chrysosporium', Microbiology, vol. 149, no. 11, pp. 3121-3127.
Jiménez-Tobon G, Kurzatkowski W, Rozbicka B, Solecka J, Pócsi I, Penninckx MJ. In situ localization of manganese peroxidase production in mycelial pallets of Phanerochaete chrysosporium. Microbiology. 2003 Nov;149(11):3121-3127.
Jiménez-Tobon, G. ; Kurzatkowski, W. ; Rozbicka, B. ; Solecka, J. ; Pócsi, I. ; Penninckx, M. J. / In situ localization of manganese peroxidase production in mycelial pallets of Phanerochaete chrysosporium. In: Microbiology. 2003 ; Vol. 149, No. 11. pp. 3121-3127.
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