In situ localization of IgG in epidermolysis bullosa acquisita by immunogold technique

S. Kárpáti, W. Stolz, M. Meurer, O. Braun-Falco, T. Krieg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Epidermolysis bullosa acquisita (EBA) is an immunologically mediated mechanobullous dermatosis. Recently, serum antibodies in patients with EBA have been reported to bind to the carboxyl terminus of type VII collagen, the disease antigen. However, the precise localization of immunoglobulins in diseased skin has not been demonstrated. Objective: In the present study skin samples taken from a 59-year-old man with EBA were processed for immunoelectron microscopy. Methods: A preembedding immunogold ultrastructural technique was applied. Results: Gold particles, indicative of IgG binding, were deposited within the anchoring plaques and within the lamina densa in some perilesional skin sections. In a similar distribution, IgG-containing amorphous aggregates were detected within the basement membrane zone of perilesional and lesional skin. In some places these immunoglobulin deposits covered the whole region of anchoring fibrils. Conclusion: These results demonstrate the precise localization of IgG within the basement membrane zone of EBA skin that corresponds to the carboxyl terminus of collagen VII in normal skin.

Original languageEnglish
Pages (from-to)726-730
Number of pages5
JournalJournal of the American Academy of Dermatology
Volume26
Issue number5 I
Publication statusPublished - 1992

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Epidermolysis Bullosa Acquisita
Immunoglobulin G
Immunohistochemistry
Skin
Skin Diseases
Basement Membrane
Immunoglobulins
Collagen Diseases
Immunoelectron Microscopy
Gold
Collagen
Antigens
Antibodies
Serum

ASJC Scopus subject areas

  • Dermatology

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In situ localization of IgG in epidermolysis bullosa acquisita by immunogold technique. / Kárpáti, S.; Stolz, W.; Meurer, M.; Braun-Falco, O.; Krieg, T.

In: Journal of the American Academy of Dermatology, Vol. 26, No. 5 I, 1992, p. 726-730.

Research output: Contribution to journalArticle

Kárpáti, S. ; Stolz, W. ; Meurer, M. ; Braun-Falco, O. ; Krieg, T. / In situ localization of IgG in epidermolysis bullosa acquisita by immunogold technique. In: Journal of the American Academy of Dermatology. 1992 ; Vol. 26, No. 5 I. pp. 726-730.
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AU - Krieg, T.

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N2 - Background: Epidermolysis bullosa acquisita (EBA) is an immunologically mediated mechanobullous dermatosis. Recently, serum antibodies in patients with EBA have been reported to bind to the carboxyl terminus of type VII collagen, the disease antigen. However, the precise localization of immunoglobulins in diseased skin has not been demonstrated. Objective: In the present study skin samples taken from a 59-year-old man with EBA were processed for immunoelectron microscopy. Methods: A preembedding immunogold ultrastructural technique was applied. Results: Gold particles, indicative of IgG binding, were deposited within the anchoring plaques and within the lamina densa in some perilesional skin sections. In a similar distribution, IgG-containing amorphous aggregates were detected within the basement membrane zone of perilesional and lesional skin. In some places these immunoglobulin deposits covered the whole region of anchoring fibrils. Conclusion: These results demonstrate the precise localization of IgG within the basement membrane zone of EBA skin that corresponds to the carboxyl terminus of collagen VII in normal skin.

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