In situ hybridization histochemistry of mRNAs for hormones and chromogranins in normal pituitary tissue and pituitary adenoma

F. Kilár, C. Muhr, K. Funa

Research output: Contribution to journalArticle

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Abstract

In situ hybridization histochemistry was employed to detect mRNAs of pituitary hormones and chromogranins in normal pituitary gland and pituitary adenomas. Oligonucleotide probes specific to the mRNAs for prolactin, growth hormone, proopiomelanocortin, the α-and β-subunits of the glycoprotein hormones and chromogranins A and B were used in the hybridization experiments. The oligonucleotides of 27 to 51 bases were labelled radioactively with dATP[α-35S] at the 3'-end using terminal deoxynucleotidyl transferase. Positive hybridization reactions were visualized by autoradiography in the normal pituitary gland with all of the probes. The clinically diagnosed pituitary adenomas (prolactinoma, acromegaly, Cushing's disease, FSH-secreting tumour) showed positive hybridization with the corresponding oligonucleotide probes. In some cases positive hybridization was also obtained with other probes, suggesting multihormone-producing character of the tumour cells. A microprolactinoma was found in a pituitary gland obtained from a patient without any known pituitary disorders. Examination of mRNAs for chromogranin A and B revealed that the normal pituitary gland contains a larger number of cells expressing chromogranin B and a lower number expressing chromogranin A and, moreover, the microprolactinoma lacked the expression of mRNA for chromogranin A but expressed that of chromogranin B.

Original languageEnglish
Pages (from-to)628-636
Number of pages9
JournalActa Endocrinologica
Volume125
Issue number6
Publication statusPublished - 1991

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Chromogranin B
Chromogranins
Chromogranin A
Pituitary Neoplasms
Pituitary Gland
Prolactinoma
In Situ Hybridization
Hormones
Messenger RNA
Oligonucleotide Probes
Pituitary Diseases
Pituitary ACTH Hypersecretion
Pro-Opiomelanocortin
Acromegaly
Pituitary Hormones
DNA Nucleotidylexotransferase
Autoradiography
Oligonucleotides
Prolactin
Growth Hormone

ASJC Scopus subject areas

  • Endocrinology

Cite this

In situ hybridization histochemistry of mRNAs for hormones and chromogranins in normal pituitary tissue and pituitary adenoma. / Kilár, F.; Muhr, C.; Funa, K.

In: Acta Endocrinologica, Vol. 125, No. 6, 1991, p. 628-636.

Research output: Contribution to journalArticle

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