In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

Jakob Toudahl Nielsen, Natalia V. Kulminskaya, Morten Bjerring, Juha M. Linnanto, Margus Rätsep, Marie Ostergaard Pedersen, Petar H. Lambrev, Márta Dorogi, Gyozo Garab, Karen Thomsen, Caroline Jegerschöld, Niels Ulrik Frigaard, Martin Lindahl, Niels Chr Nielsen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba.Tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

Original languageEnglish
Article number12454
JournalNature communications
Volume7
DOIs
Publication statusPublished - Aug 18 2016

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Nielsen, J. T., Kulminskaya, N. V., Bjerring, M., Linnanto, J. M., Rätsep, M., Pedersen, M. O., Lambrev, P. H., Dorogi, M., Garab, G., Thomsen, K., Jegerschöld, C., Frigaard, N. U., Lindahl, M., & Nielsen, N. C. (2016). In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum. Nature communications, 7, [12454]. https://doi.org/10.1038/ncomms12454