In situ ellipsometric study of surface immobilization of flagellar filaments

S. Kurunczi, A. Németh, T. Hülber, P. Kozma, P. Petrik, H. Jankovics, A. Sebestyén, F. Vonderviszt, M. Fried, I. Bársony

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Protein filaments composed of thousands of subunits are promising candidates as sensing elements in biosensors. In this work in situ spectroscopic ellipsometry is applied to monitor the surface immobilization of flagellar filaments. This study is the first step towards the development of layers of filamentous receptors for sensor applications. Surface activation is performed using silanization and a subsequent glutaraldehyde crosslinking. Structure of the flagellar filament layers immobilized on activated and non-activated Si wafer substrates is determined using a two-layer effective medium model that accounted for the vertical density distribution of flagellar filaments with lengths of 300-1500 nm bound to the surface. The formation of the first interface layer can be explained by the multipoint covalent attachment of the filaments, while the second layer is mainly composed of tail pinned filaments floating upwards with the free parts. As confirmed by atomic force microscopy, covalent immobilization resulted in an increased surface density compared to absorption.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalApplied Surface Science
Volume257
Issue number1
DOIs
Publication statusPublished - Oct 15 2010

Keywords

  • Biosensor
  • Ellipsometry
  • Flagellar filament
  • Protein immobilization

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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