In-situ clean-up and oplc fractionation of chamomile flower extract to search active components by bioautography

E. Mincsovics, P. Ott, Agnes Alberti, Andrea Böszörményi, Eva B. Héthelyi, E. Szőke, A. Kéry, E. Lemberkovics, A. Móricz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Bioassay-guided isolation of antibacterial components of chamomile flower methanol extract was performed by overpressured layer chromatography (OPLC) with on-line detection, fractionation combined with sample clean-up in-situ in the adsorbent bed after off-line sample application. The antibacterial effect of the eluted fractions and of those compounds remaining on the adsorbent layer after separation was tested with direct bioautography (DB) against the bioluminescent Pseudomonas savastanoi pv. maculicola and Vibrio fischeri. The fractions with high biological activity were analyzed by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) and liquid chromatography and tandem mass spectrometry (LC-MS/MS). Two active uneluted compounds were characterized by off-line OPLC-MS using a thin-layer chromatography (TLC)-MS interface. Mainly, essential oil components, coumarins, flavonoids, phenolic acids, and fatty acids were identified in the active fractions.

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalJournal of Planar Chromatography - Modern TLC
Volume26
Issue number2
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Chamomile
Fractionation
Chromatography
Adsorbents
Mass spectrometry
Aliivibrio fischeri
Coumarins
Solid Phase Microextraction
Thin layer chromatography
Bioassay
Liquid chromatography
Volatile Oils
Thin Layer Chromatography
Tandem Mass Spectrometry
Pseudomonas
Bioactivity
Flavonoids
Liquid Chromatography
Biological Assay
Gas chromatography

Keywords

  • Antibacterial activity
  • Chamomile (Matricaria recutita L.)
  • Combined on-line/off-line OPLC
  • GC-MS
  • HPLC-MS/MS
  • Off-line OPLC-MS
  • Overpressured layer chromatography OPLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

In-situ clean-up and oplc fractionation of chamomile flower extract to search active components by bioautography. / Mincsovics, E.; Ott, P.; Alberti, Agnes; Böszörményi, Andrea; Héthelyi, Eva B.; Szőke, E.; Kéry, A.; Lemberkovics, E.; Móricz, A.

In: Journal of Planar Chromatography - Modern TLC, Vol. 26, No. 2, 04.2013, p. 172-179.

Research output: Contribution to journalArticle

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