In silico analysis of a putative SPIRAL gene related to strawberry ripening

D. Polgári, B. Kalapos, V. Tisza, L. Kovács, B. Kerti, L. Heszky, E. Kiss

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Abstract

The aim of this study was to characterize a gene associated with ripening in strawberry, a non-climacteric fruit. Differently expressed transcripts of candidate genes functioning in fruit development and ripening were identified from strawberry ( Fragaria × ananassa Duch.) in four ripening stages using the cDNA-AFLP method. The cDNA fragment designated C11M32M003 was selected from the putative ripening-related genes for further analysis. This transcript accumulated in the green receptacle, and the achene, but gene expression decreased in both tissues in parallel with the progress of ripening (Balogh, 2006). In silico analysis revealed that both the cDNA-AFLP fragment (C11M32M003) and the full-length cDNA AY695666 showed over 60% homology at the nucleotide level with two gene groups found in various plant species, including Arabidopsis thaliana . One of the candidate groups consisted of NITRILASE sequences thought to be related to auxin biosynthesis. As an alternative, a lesser known gene group named SPIRAL was suggested. The results of the detailed bioinformatic comparisons presented in this paper prove that the strawberry sequence analysed belongs to the SPIRAL gene family.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalActa Agronomica Hungarica
Volume58
Issue number3
DOIs
Publication statusPublished - Sep 1 2010

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Keywords

  • Fragaria × ananassa Duch.
  • SPIRAL and NITRILASE genes
  • in silico analysis
  • non-climacteric ripening

ASJC Scopus subject areas

  • Agronomy and Crop Science

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