Különbözo ploidszintü es genomösszetetelü Triticum fajok noi gametofitonjának differenciälodäsa természetes es in vitro körülmenyek között

Translated title of the contribution: In planta and in vitro differentiation of the female gametophytes of various Triticum species with different ploidy levels and genome compositions

B. Barnabás, Ponya Zsolt

Research output: Contribution to journalArticle

Abstract

Comparisons were made on the in vivo and in vitro development of the female gametophytes of three Triticum species with different ploidy levels and genome compositions (T. aestivum cv. Mv. 15. 6n. T. araraticum, 4n and T. monococcutn, 2n). The development of embryo sacs in in vitro spikelet cultures is similar to that of the in planta control in all three species, though there were differences in their size. The dynamics of differentiation was influenced by the ploidy level; the differentiation of the 7-cellular female gametophyte took a day longer in the tetraploid and diploid species than in the hexaploid species. For the species examined the embryo sac was formed from the functional marcrospore in 3-4 days due to three mitotic divisions in rapid succession, while the trinucleate pollen, differentiated in the course of 2 microspore mitotic divisions, could not be observed until the 7th day, thus confirming the presence of protogyny in Triticum species. The differing ploidy levels of the species examined were also exhibited in the size of the embryo sac. The embryo sac of the hexaploid species was significantly larger than those of the tetraploid and diploid species. The female gametophytes reached their maximum size 2-3 days before the male gametophytes matured. These observations suggest that further studies will be required to clarify when gametophytes can be regarded as functionally mature and which phase of gametophytic development is best suited for cell manipulation.

Original languageHungarian
Pages (from-to)125-131
Number of pages7
JournalNovenytermeles
Volume46
Issue number2
Publication statusPublished - 1997

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embryo sac
gametophytes
Triticum
Plantae
ploidy
genome
hexaploidy
tetraploidy
diploidy
protogyny
microspores
Triticum aestivum
inflorescences
pollen
cells

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "K{\"u}l{\"o}nb{\"o}zo ploidszint{\"u} es genom{\"o}sszetetel{\"u} Triticum fajok noi gametofitonj{\'a}nak differenci{\"a}lod{\"a}sa term{\'e}szetes es in vitro k{\"o}r{\"u}lmenyek k{\"o}z{\"o}tt",
abstract = "Comparisons were made on the in vivo and in vitro development of the female gametophytes of three Triticum species with different ploidy levels and genome compositions (T. aestivum cv. Mv. 15. 6n. T. araraticum, 4n and T. monococcutn, 2n). The development of embryo sacs in in vitro spikelet cultures is similar to that of the in planta control in all three species, though there were differences in their size. The dynamics of differentiation was influenced by the ploidy level; the differentiation of the 7-cellular female gametophyte took a day longer in the tetraploid and diploid species than in the hexaploid species. For the species examined the embryo sac was formed from the functional marcrospore in 3-4 days due to three mitotic divisions in rapid succession, while the trinucleate pollen, differentiated in the course of 2 microspore mitotic divisions, could not be observed until the 7th day, thus confirming the presence of protogyny in Triticum species. The differing ploidy levels of the species examined were also exhibited in the size of the embryo sac. The embryo sac of the hexaploid species was significantly larger than those of the tetraploid and diploid species. The female gametophytes reached their maximum size 2-3 days before the male gametophytes matured. These observations suggest that further studies will be required to clarify when gametophytes can be regarded as functionally mature and which phase of gametophytic development is best suited for cell manipulation.",
author = "B. Barnab{\'a}s and Ponya Zsolt",
year = "1997",
language = "Hungarian",
volume = "46",
pages = "125--131",
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T1 - Különbözo ploidszintü es genomösszetetelü Triticum fajok noi gametofitonjának differenciälodäsa természetes es in vitro körülmenyek között

AU - Barnabás, B.

AU - Zsolt, Ponya

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N2 - Comparisons were made on the in vivo and in vitro development of the female gametophytes of three Triticum species with different ploidy levels and genome compositions (T. aestivum cv. Mv. 15. 6n. T. araraticum, 4n and T. monococcutn, 2n). The development of embryo sacs in in vitro spikelet cultures is similar to that of the in planta control in all three species, though there were differences in their size. The dynamics of differentiation was influenced by the ploidy level; the differentiation of the 7-cellular female gametophyte took a day longer in the tetraploid and diploid species than in the hexaploid species. For the species examined the embryo sac was formed from the functional marcrospore in 3-4 days due to three mitotic divisions in rapid succession, while the trinucleate pollen, differentiated in the course of 2 microspore mitotic divisions, could not be observed until the 7th day, thus confirming the presence of protogyny in Triticum species. The differing ploidy levels of the species examined were also exhibited in the size of the embryo sac. The embryo sac of the hexaploid species was significantly larger than those of the tetraploid and diploid species. The female gametophytes reached their maximum size 2-3 days before the male gametophytes matured. These observations suggest that further studies will be required to clarify when gametophytes can be regarded as functionally mature and which phase of gametophytic development is best suited for cell manipulation.

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