In old age the majority of thyroid peroxidase autoantibodies are directed to a single TPO domain irrespective of thyroid function and iodine intake

Barbara Czarnocka, I. Szabolcs, Danuta Pastuszko, Joachim Feldkamp, O. Dohán, Jan Podoba, Bjorn Wenzel

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: We have examined (1) which epitopes on thyroid peroxidase (TPO) are recognized by TPO auto-antibodies (TPO-Aab) in old age and to what extent? (2) Does the TPO-Aab pattern differ in euthyroid and hypothyroid elderly subjects or does it depend on their iodine intake? DESIGN: TPO-Aab positive sera obtained form a screening study of nursing-home residents living in areas of varying iodine intake were tested by competition studies with monoclonal antibodies (mAbs) recognizing different epitopes on TPO. SUBJECTS: The nursing-home residents with TPO-Aab positivity were from (A) an iodine abundant area (Eastern Hungary median iodine excretion -MIE-: 0·462 μmol/mmol creatine, N = 13); (B) an area of obligatory iodinated salt prophylaxis since the 1950s (Slovakia, MIE: 0·090 μmol/mmol creatine, N = 11); (C) a moderately iodine-deficient area (Northern Hungary, MIE: 0.065 μmol/mmol creatinine, N = 13). MEASUREMENTS: Thirteen murine TPO antibodies generated against several epitopes of the four (A, B, C, D) antigenic domains on the TPO were co-incubated with the TPO-Aab positive sera on TPO coated microtitre plates. The amount of mAb bound was estimated after further incubation with goat anti-mouse antibodies, conjugated with horseradish peroxidase and tetramethylbenzidine as chromogen. The TPO-Aab positive sera were characterized by the pattern of percentage of inhibition of mAb binding caused by the TPO-Aabs. RESULTS: TPO-Aabs inhibited only the binding of mAbs raised against the antigenic domains A (mAb9, mAb2, mAb60) and B (mAb64, mAb59, mAb18, mAb15). The extent of inhibition depended upon the TPO-Aab titre but in all cases the binding of mAb9 was inhibited to the highest degree. The percentage inhibition of mAb9 was (a) 34 ± 17% (M ± SD) caused by sera (N = 8) with TPO-Aab titre 1/100-1/200 (higher than that of all mAbs recognizing domain B, P <0.01-P > 0.001), (b) 76 ± 18% caused by sera (N = 14) with TPO-Aab titre 1/1000 (higher than that of all other mAbs-P

Original languageEnglish
Pages (from-to)803-808
Number of pages6
JournalClinical Endocrinology
Volume48
Issue number6
DOIs
Publication statusPublished - 1998

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Iodide Peroxidase
Iodine
Autoantibodies
Thyroid Gland
Monoclonal Antibodies
Epitopes
Serum
Creatine
Hungary
Nursing Homes
Slovakia
Antibodies
Horseradish Peroxidase

ASJC Scopus subject areas

  • Endocrinology

Cite this

In old age the majority of thyroid peroxidase autoantibodies are directed to a single TPO domain irrespective of thyroid function and iodine intake. / Czarnocka, Barbara; Szabolcs, I.; Pastuszko, Danuta; Feldkamp, Joachim; Dohán, O.; Podoba, Jan; Wenzel, Bjorn.

In: Clinical Endocrinology, Vol. 48, No. 6, 1998, p. 803-808.

Research output: Contribution to journalArticle

Czarnocka, Barbara ; Szabolcs, I. ; Pastuszko, Danuta ; Feldkamp, Joachim ; Dohán, O. ; Podoba, Jan ; Wenzel, Bjorn. / In old age the majority of thyroid peroxidase autoantibodies are directed to a single TPO domain irrespective of thyroid function and iodine intake. In: Clinical Endocrinology. 1998 ; Vol. 48, No. 6. pp. 803-808.
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T1 - In old age the majority of thyroid peroxidase autoantibodies are directed to a single TPO domain irrespective of thyroid function and iodine intake

AU - Czarnocka, Barbara

AU - Szabolcs, I.

AU - Pastuszko, Danuta

AU - Feldkamp, Joachim

AU - Dohán, O.

AU - Podoba, Jan

AU - Wenzel, Bjorn

PY - 1998

Y1 - 1998

N2 - OBJECTIVE: We have examined (1) which epitopes on thyroid peroxidase (TPO) are recognized by TPO auto-antibodies (TPO-Aab) in old age and to what extent? (2) Does the TPO-Aab pattern differ in euthyroid and hypothyroid elderly subjects or does it depend on their iodine intake? DESIGN: TPO-Aab positive sera obtained form a screening study of nursing-home residents living in areas of varying iodine intake were tested by competition studies with monoclonal antibodies (mAbs) recognizing different epitopes on TPO. SUBJECTS: The nursing-home residents with TPO-Aab positivity were from (A) an iodine abundant area (Eastern Hungary median iodine excretion -MIE-: 0·462 μmol/mmol creatine, N = 13); (B) an area of obligatory iodinated salt prophylaxis since the 1950s (Slovakia, MIE: 0·090 μmol/mmol creatine, N = 11); (C) a moderately iodine-deficient area (Northern Hungary, MIE: 0.065 μmol/mmol creatinine, N = 13). MEASUREMENTS: Thirteen murine TPO antibodies generated against several epitopes of the four (A, B, C, D) antigenic domains on the TPO were co-incubated with the TPO-Aab positive sera on TPO coated microtitre plates. The amount of mAb bound was estimated after further incubation with goat anti-mouse antibodies, conjugated with horseradish peroxidase and tetramethylbenzidine as chromogen. The TPO-Aab positive sera were characterized by the pattern of percentage of inhibition of mAb binding caused by the TPO-Aabs. RESULTS: TPO-Aabs inhibited only the binding of mAbs raised against the antigenic domains A (mAb9, mAb2, mAb60) and B (mAb64, mAb59, mAb18, mAb15). The extent of inhibition depended upon the TPO-Aab titre but in all cases the binding of mAb9 was inhibited to the highest degree. The percentage inhibition of mAb9 was (a) 34 ± 17% (M ± SD) caused by sera (N = 8) with TPO-Aab titre 1/100-1/200 (higher than that of all mAbs recognizing domain B, P <0.01-P > 0.001), (b) 76 ± 18% caused by sera (N = 14) with TPO-Aab titre 1/1000 (higher than that of all other mAbs-P

AB - OBJECTIVE: We have examined (1) which epitopes on thyroid peroxidase (TPO) are recognized by TPO auto-antibodies (TPO-Aab) in old age and to what extent? (2) Does the TPO-Aab pattern differ in euthyroid and hypothyroid elderly subjects or does it depend on their iodine intake? DESIGN: TPO-Aab positive sera obtained form a screening study of nursing-home residents living in areas of varying iodine intake were tested by competition studies with monoclonal antibodies (mAbs) recognizing different epitopes on TPO. SUBJECTS: The nursing-home residents with TPO-Aab positivity were from (A) an iodine abundant area (Eastern Hungary median iodine excretion -MIE-: 0·462 μmol/mmol creatine, N = 13); (B) an area of obligatory iodinated salt prophylaxis since the 1950s (Slovakia, MIE: 0·090 μmol/mmol creatine, N = 11); (C) a moderately iodine-deficient area (Northern Hungary, MIE: 0.065 μmol/mmol creatinine, N = 13). MEASUREMENTS: Thirteen murine TPO antibodies generated against several epitopes of the four (A, B, C, D) antigenic domains on the TPO were co-incubated with the TPO-Aab positive sera on TPO coated microtitre plates. The amount of mAb bound was estimated after further incubation with goat anti-mouse antibodies, conjugated with horseradish peroxidase and tetramethylbenzidine as chromogen. The TPO-Aab positive sera were characterized by the pattern of percentage of inhibition of mAb binding caused by the TPO-Aabs. RESULTS: TPO-Aabs inhibited only the binding of mAbs raised against the antigenic domains A (mAb9, mAb2, mAb60) and B (mAb64, mAb59, mAb18, mAb15). The extent of inhibition depended upon the TPO-Aab titre but in all cases the binding of mAb9 was inhibited to the highest degree. The percentage inhibition of mAb9 was (a) 34 ± 17% (M ± SD) caused by sera (N = 8) with TPO-Aab titre 1/100-1/200 (higher than that of all mAbs recognizing domain B, P <0.01-P > 0.001), (b) 76 ± 18% caused by sera (N = 14) with TPO-Aab titre 1/1000 (higher than that of all other mAbs-P

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