In-depth characterization and computational 3D reconstruction of flagellar filament protein layer structure based on in situ spectroscopic ellipsometry measurements

Peter Kozma, Daniel Kozma, Andrea Nemeth, Hajnalka Jankovics, Sandor Kurunczi, Robert Horvath, Ferenc Vonderviszt, Miklos Fried, Peter Petrik

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study, we have reconstructed the statistical 3D structure of hundreds of nanometers thick surface immobilized flagellar filament protein layers in their native environment, in buffer solution. The protein deposition onto the surface activated Ta 2 O 5 film was performed in a flow cell, and the immobilization process was followed by in situ spectroscopic ellipsometry. A multilayer optical model was developed, in that the protein layer was described by five effective medium sublayers. Applying this method, an in-depth analysis of the protein layer formation was performed. Based on the kinetics in the distribution of the surface mass density, the statistical properties of the filamentous film could be determined computationally as a function of the measurement time. It was also demonstrated that the 3D structure of the protein layer can be reconstructed based on the calculated in-depth mass density profile. The computational investigation revealed that the filaments can be classified into two individual groups in approximately equal ratio according to their orientation. In the first group the filaments are close to laying position, whereas in the second group they are in a standing position, resulting in a significantly denser sublayer close to the substrate than at a larger distance.

Original languageEnglish
Pages (from-to)7160-7166
Number of pages7
JournalApplied Surface Science
Volume257
Issue number16
DOIs
Publication statusPublished - Jun 1 2011

Keywords

  • 3D layer structure reconstruction
  • In situ process control
  • In-depth distribution
  • Mass density profile
  • Protein layer structure
  • Spectroscopic ellipsometry

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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