Improving selenium extraction by sequential enzymatic processes for Se-speciation of selenium-enriched Agaricus bisporus

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62 Citations (Scopus)

Abstract

Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 μg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes - Lysing enzyme and Driselase - in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestion. A 3-step procedure applying Lysing enzyme and pronase gave the highest extraction efficiency (89%); however, the best species recovery was achieved by a one-step proteolytic procedure. All the procedures of selenium speciation were controlled by independent ICP-AES analysis measuring the total amount of selenium.

Original languageEnglish
Pages (from-to)473-480
Number of pages8
JournalFresenius' journal of analytical chemistry
Volume372
Issue number3
Publication statusPublished - 2002

Fingerprint

Agaricus
Selenium
Cell Wall
Cells
Enzymes
Proteolysis
Pronase
Agaricales
Yeast
Digestion
Soil
Yeasts
Recovery
Experiments

Keywords

  • Cell wall digestion
  • Se-enriched Agaricus bisporus
  • Selenium speciation
  • Sequential enzymatic sample preparation

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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abstract = "Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 μg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes - Lysing enzyme and Driselase - in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestion. A 3-step procedure applying Lysing enzyme and pronase gave the highest extraction efficiency (89{\%}); however, the best species recovery was achieved by a one-step proteolytic procedure. All the procedures of selenium speciation were controlled by independent ICP-AES analysis measuring the total amount of selenium.",
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AU - Dernovics, M.

AU - Stefánka, Z.

AU - Fodor, P.

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N2 - Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 μg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes - Lysing enzyme and Driselase - in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestion. A 3-step procedure applying Lysing enzyme and pronase gave the highest extraction efficiency (89%); however, the best species recovery was achieved by a one-step proteolytic procedure. All the procedures of selenium speciation were controlled by independent ICP-AES analysis measuring the total amount of selenium.

AB - Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 μg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes - Lysing enzyme and Driselase - in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestion. A 3-step procedure applying Lysing enzyme and pronase gave the highest extraction efficiency (89%); however, the best species recovery was achieved by a one-step proteolytic procedure. All the procedures of selenium speciation were controlled by independent ICP-AES analysis measuring the total amount of selenium.

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