Improving post-thaw survival of cryopreserved mouse blastocysts by hydrostatic pressure challenge

Csaba Pribenszky, Miklós Molnár, Sándor Cseh, László Solti

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43 Citations (Scopus)

Abstract

The purpose of the experiments was to study the effect of high hydrostatic pressure treatment prior to vitrification to the survival of expanded mouse blastocysts. High hydrostatic pressure has been reported to induce the production of "shock proteins" in bacteria, which can provide a possibility of cross-protection to other environmental stresses. The possible beneficial effects of this alleged principle was examined on embryo vitrification. First, the behaviour of blastocysts was studied at altered pressure conditions. In the second part of the study, pressure treatment was combined with a cryopreservation protocol. Our results indicate that the survival of pressurized mouse embryos depends on the magnitude and the duration of pressure applied. We demonstrated that a preceding pressure treatment strikingly increases the survival of the frozen blastocysts as well as the speed of resumption of the development, and hatching rate.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalAnimal Reproduction Science
Volume87
Issue number1-2
DOIs
Publication statusPublished - Jun 2005

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Keywords

  • Blastocyst
  • Cross-protection
  • Cryopreservation
  • Embryo
  • Hydrostatic pressure

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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