Improvement of the electron microscopic detection of peroxidase activity by means of the silver intensification of the diaminobenzidine reaction in the rat nervous system

Zsolt Liposits, Tamás Görcs, Ferenc Gallyas, Béla Kosaras, György Sétáló

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

For the detection of the peroxidase activity at the electron microscopic level, a recently developed post-intensification method is applied, which plates metallic gold onto the end-product of the diaminobenzidine (DAE) reaction. Ultrastructural analysis of rat hypoglossal neurons labeled with horseradish peroxidase (HRP) through axonal transport reveals that the method is highly specific and more sensitive than the classical HRPDABOsO4 sequence. Gold grains of 2-15 nm in diameter are present in the HRP-containing organelles of the neuron, whereas other elements of the brain tissue do not contain metallic gold.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalNeuroscience Letters
Volume31
Issue number1
DOIs
Publication statusPublished - Jul 20 1982

Keywords

  • diaminobenzidine end-product
  • electron microscopy
  • horseradish peroxidase labeling
  • rat
  • silver intensification
  • thioglycolic acid

ASJC Scopus subject areas

  • Neuroscience(all)

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