A fertozo betegségek diagnosztikai mödszereinek tökéletesítése

Translated title of the contribution: Improvement of diagnostic methods for infectious diseases

Research output: Contribution to journalArticle

Abstract

Diagnostic methods to be used in case of notifiable infectious diseases have been regulated partly by regulations and partly by guidelines to be considered obligatory. The use of internationally accepted standard methods is especially important because the veterinary authorities issue different transport papers, certificates based on the results of these investigations. Only with a few exceptions, the diagnosis of infectious diseases in the list A of Office International des Epizootics is the responsibility of central veterinary laboratories in each country. The laboratory procedures are based on the demonstration of virus its antigen or nucleic acid in the animal tissues, isolation of virus, as well as demonstration of antibodies developing in the blood serum by different methods. ELISA and virus isolation are used for the demonstration of foot-and-mouth disease (FMD) virus. Serum antibodies are demonstrated by virus neutralization or ELISA tests. In case of swine vesicular disease (SVD) the samples are tested parallel also for the presence of FMD virus. The SVD virus can be isolated in cell cultures of porcine origin and can not be isolated in those of bovine origin. Serum antibodies are demonstrated by virus neutralization and ELISA tests. Immunofluorescent investigation of tissue samples and virus isolation methods are used for the diagnosis of classical swine fever (CSF). Monoclonal antibodies are used to differentiate among CSF, BVD virus and Suvac vaccine virus strains. Different ELISA and virus neutralization test are used for the demonstration of serum antibodies. In case of a suspected African Swine Fever case, haemadsorption and immunofluorescent tests are used for the demonstration of the virus and also pigs are inoculated with the suspected material. ELISA is used for the serological diagnosis of EBL. Cultivation of avian influenza viruses is carried out in embryonated eggs. The type of virus is determined by immuno-diffusion test and the type of haemagglutinin and neuraminidase - and occasionally - the virulence have also been determined. Haemagglutination inhibition test is used for the demorstration of seaim antibodies. Practically the same methods are used for the diagnosis of Newcastle disease.

Original languageHungarian
Pages (from-to)686-692
Number of pages7
JournalMagyar Allatorvosok Lapja
Volume119
Issue number11
Publication statusPublished - 1997

Fingerprint

diagnostic techniques
infectious diseases
Communicable Diseases
Viruses
viruses
blood serum
enzyme-linked immunosorbent assay
neutralization tests
antibodies
Enzyme-Linked Immunosorbent Assay
hog cholera
Foot-and-mouth disease virus
Antibodies
Foot-and-Mouth Disease Virus
Neutralization Tests
Serum
swine vesicular disease
Enterovirus B
Swine Vesicular Disease
African swine fever

ASJC Scopus subject areas

  • veterinary(all)

Cite this

A fertozo betegségek diagnosztikai mödszereinek tökéletesítése. / Vilmos, Pálfi; Lajos, Tekes; János, Tanyi.

In: Magyar Allatorvosok Lapja, Vol. 119, No. 11, 1997, p. 686-692.

Research output: Contribution to journalArticle

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