Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: Process optimization by experimental design approach

Gopalakrishnan Kumar, Péter Bakonyi, Periyasamy Sivagurunathan, Nándor Nemestóthy, Katalin Bélafi-Bakó, Chiu Yue Lin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock pretreatment (95 °C, 30 min) was employed. Afterwards, an experimental design was applied to find the optimal conditions for H2 production using heat-pretreated seed culture. The optimal substrate concentration, pH and temperature were determined by using response surface methodology as 205 g/L, 6.53 and 55.1 °C, respectively. Under these circumstances, the highest HPR of 1.36 L H2/L-d was predicted. Verification tests proved the reliability of the statistical approach. As a result of the heat pretreatment and fermentation optimization, a significant (~ 4 folds) increase in HPR was achieved. PCR-DGGE results revealed that Clostridium sp. were majorly present under the optimal conditions.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalBiofuel Research Journal
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • Biohydrogen
  • De-oiled Jatropha waste
  • Experimental design
  • Inoculum pretreatment
  • Optimization
  • Pretreatment

ASJC Scopus subject areas

  • Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology

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