Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: Process optimization by experimental design approach

Gopalakrishnan Kumar, Péter Bakonyi, Periyasamy Sivagurunathan, N. Nemestóthy, K. Bélafi-Bakó, Chiu Yue Lin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock pretreatment (95 °C, 30 min) was employed. Afterwards, an experimental design was applied to find the optimal conditions for H2 production using heat-pretreated seed culture. The optimal substrate concentration, pH and temperature were determined by using response surface methodology as 205 g/L, 6.53 and 55.1 °C, respectively. Under these circumstances, the highest HPR of 1.36 L H2/L-d was predicted. Verification tests proved the reliability of the statistical approach. As a result of the heat pretreatment and fermentation optimization, a significant (~ 4 folds) increase in HPR was achieved. PCR-DGGE results revealed that Clostridium sp. were majorly present under the optimal conditions.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalBiofuel Research Journal
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Jatropha
Hydrogen production
Design of experiments
Hydrogen
Research Design
Sewage
Clostridium
Hot Temperature
Sewage sludge
Fermentation
Seed
Thermogenesis
Shock
Seeds
Substrates
Polymerase Chain Reaction
Temperature

Keywords

  • Biohydrogen
  • De-oiled Jatropha waste
  • Experimental design
  • Inoculum pretreatment
  • Optimization
  • Pretreatment

ASJC Scopus subject areas

  • Biotechnology
  • Fuel Technology
  • Renewable Energy, Sustainability and the Environment

Cite this

Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment : Process optimization by experimental design approach. / Kumar, Gopalakrishnan; Bakonyi, Péter; Sivagurunathan, Periyasamy; Nemestóthy, N.; Bélafi-Bakó, K.; Lin, Chiu Yue.

In: Biofuel Research Journal, Vol. 2, No. 1, 01.01.2015, p. 209-214.

Research output: Contribution to journalArticle

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