Improved isolation protocol to detect high molecular weight polysaccharide structures of Campylobacter jejuni

Judit K. Kovács, Péter Felso, L. Emődy, G. Schneider, Béla Kocsis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Simple detection of high molecular weight, LPS-like structures of Campylobacter jejuni is still an unsolved problem. A phenol-free extraction method for the detection of HMW polysaccharide was developed without the need for Western blot. This method provides a reliable technique for large-scale screening and comparative characterization study of different isolates.

Original languageEnglish
Pages (from-to)55-57
Number of pages3
JournalJournal of Microbiological Methods
Volume107
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

Campylobacter jejuni
Polysaccharides
Molecular Weight
Phenol
Western Blotting

Keywords

  • Campylobacter jejuni
  • LPS
  • Polysaccharide
  • Silver staining

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)
  • Medicine(all)

Cite this

Improved isolation protocol to detect high molecular weight polysaccharide structures of Campylobacter jejuni. / Kovács, Judit K.; Felso, Péter; Emődy, L.; Schneider, G.; Kocsis, Béla.

In: Journal of Microbiological Methods, Vol. 107, 01.12.2014, p. 55-57.

Research output: Contribution to journalArticle

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