Improved growth of cultured brain microvascular endothelial cells on glass coated with a biological matrix

E. Dömötör, I. Sipos, A. Kittel, N. J. Abbott, V. Ádám-Vizi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

An improved method for culturing primary rat brain capillary endothelial cells on glass has been developed, using a corneal extracellular matrix coat. Since the collagen-coated plastic attachment surface conventionally used for primary cultures of brain microvascular endothelium gives a high level of background fluorescence in microfluorimetric studies, an alternative attachment surface was tested involving no plastic element. Five substrata combinations were examined and a new combination of glass and corneal endothelial extracellular matrix coat was found to provide excellent cell adhesion, culture growth and purity. Other established substrata combinations tested for comparison, either involved plastic, or used glass with collagen or carbodiimide and collagen coating but the last two gave poor endothelial cell adhesion and growth. Our method using this new attachment surface combination results in stable and pure endothelial cultures, as verified by immunocytochemistry, which are suitable for fluorimetric investigations.

Original languageEnglish
Pages (from-to)473-478
Number of pages6
JournalNeurochemistry International
Volume33
Issue number6
DOIs
Publication statusPublished - 1998

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Plastics
Glass
Collagen
Endothelial Cells
Cell Adhesion
Extracellular Matrix
Brain
Growth
Carbodiimides
Endothelium
Cell Culture Techniques
Fluorescence
Immunohistochemistry

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Improved growth of cultured brain microvascular endothelial cells on glass coated with a biological matrix. / Dömötör, E.; Sipos, I.; Kittel, A.; Abbott, N. J.; Ádám-Vizi, V.

In: Neurochemistry International, Vol. 33, No. 6, 1998, p. 473-478.

Research output: Contribution to journalArticle

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