Implementation of an experimental isolated lung perfusion model on surgically resected human lobes

Alexis Slama, Christian Raber, Celia Hedderich, Paul Stockhammer, B. Hegedűs, Achim Koch, Dirk Theegarten, Till Ploenes, Clemens Aigner

Research output: Contribution to journalArticle

Abstract

Isolated lung perfusion (ILP) is an ideal model to study treatment effects on a variety of pathologies. As published research mostly relies on rejected donor lungs or animal organs, this study investigates the use of surgically resected human lobes as an alternative and novel model for personalized experimental research. Ten surgically resected lobes were perfused in acellular and normothermic condition. The indication for surgery was lung cancer. Perfusion and ventilation were adapted to the size of the lobes and both functional and metabolic parameters were assessed during ILP. Patients (age 67.5 y (59–81)|♀n = 3|♂n = 7) underwent anatomic pulmonary lobectomy. Ischemic time between arterial ligation and ILP was 226 minutes (161–525). Median duration of ILP was 135 (87–366) minutes. Gas exchange and mechanical respiratory parameters remained steady during ILP (pulmonary venous pO2 196(151–219) mmHg | peak AWP: 14.5(11–22) cmH2O). Metabolism stayed constant during ILP (Glucose consumption: 1.86 mg/min/LTLC (95%CI: −2.09 to −1.63) | lactate production: 0.005 mmol/min/ LTLC (95%CI: 0.004 to 0.007)). ILP of surgically resected human lobes is a feasible and promising method. By maintaining a near physiological setting, this model may pave the way for future experimental lung research including cancer research, transplantation, physiology, pharmacology and mechanical ventilation.

Original languageEnglish
Article number12193
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Perfusion
Lung
Research
Animal Structures
Artificial Respiration
Ligation
Ventilation
Lactic Acid
Lung Neoplasms
Theoretical Models
Transplantation
Gases
Tissue Donors
Pharmacology
Pathology
Glucose

ASJC Scopus subject areas

  • General

Cite this

Implementation of an experimental isolated lung perfusion model on surgically resected human lobes. / Slama, Alexis; Raber, Christian; Hedderich, Celia; Stockhammer, Paul; Hegedűs, B.; Koch, Achim; Theegarten, Dirk; Ploenes, Till; Aigner, Clemens.

In: Scientific reports, Vol. 9, No. 1, 12193, 01.12.2019.

Research output: Contribution to journalArticle

Slama, A, Raber, C, Hedderich, C, Stockhammer, P, Hegedűs, B, Koch, A, Theegarten, D, Ploenes, T & Aigner, C 2019, 'Implementation of an experimental isolated lung perfusion model on surgically resected human lobes', Scientific reports, vol. 9, no. 1, 12193. https://doi.org/10.1038/s41598-019-48719-8
Slama, Alexis ; Raber, Christian ; Hedderich, Celia ; Stockhammer, Paul ; Hegedűs, B. ; Koch, Achim ; Theegarten, Dirk ; Ploenes, Till ; Aigner, Clemens. / Implementation of an experimental isolated lung perfusion model on surgically resected human lobes. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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