Impedimetric aflatoxin M1 immunosensor based on colloidal gold and silver electrodeposition

Attila Vig, Antonio Radoi, Xavier Muñoz-Berbel, G. Gyémánt, Jean Louis Marty

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

An impedimetric immunosensor based on colloidal gold and silver electrodeposition for the detection of aflatoxin M1 (AFM1) herein is reported. An indirect like competitive ELISA procedure was performed on screen-printed electrodes (SPEs) in presence of anti-AFM1 gold-labelled antibodies. Silver was chronoamperometrically electrodeposited at a fixed applied potential and for a determined period of time to amplify the signal. The calculated charge transfer resistance (Rct) was found to correlate well with the concentration of AFM1. The linear working range of the described AFM1 immunosensor ranged between 15 and 1000 ng/L with a limit of detection (LoD) equal to 15 ng/L (R.S.D. = 20%). Impedimetric results were confronted with linear sweep voltametry (LSV) and corresponded well to this technique.

Original languageEnglish
Pages (from-to)214-220
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume138
Issue number1
DOIs
Publication statusPublished - Apr 24 2009

Fingerprint

Aflatoxin M1
Aflatoxins
Immunosensors
Gold Colloid
Electrodeposition
electrodeposition
Silver
Gold
silver
gold
antibodies
charge transfer
electrodes
Antibodies
Charge transfer
Electrodes
colloidal silver

Keywords

  • AFM
  • Immunosensor
  • Impedance
  • Silver electrodeposition

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Impedimetric aflatoxin M1 immunosensor based on colloidal gold and silver electrodeposition. / Vig, Attila; Radoi, Antonio; Muñoz-Berbel, Xavier; Gyémánt, G.; Marty, Jean Louis.

In: Sensors and Actuators, B: Chemical, Vol. 138, No. 1, 24.04.2009, p. 214-220.

Research output: Contribution to journalArticle

Vig, Attila ; Radoi, Antonio ; Muñoz-Berbel, Xavier ; Gyémánt, G. ; Marty, Jean Louis. / Impedimetric aflatoxin M1 immunosensor based on colloidal gold and silver electrodeposition. In: Sensors and Actuators, B: Chemical. 2009 ; Vol. 138, No. 1. pp. 214-220.
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